From 2014.igem.org
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Revision as of 00:51, 18 October 2014
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Welcome!
Team Jilin_China
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Top |
May to July, 2014
- MlrA gene coding optimization（codon of lactic acid bacteria）
- MlrA gene synthesis(synthesis by pieces)
- Sequence analysis and discussion about whether it can be split
- Synthetic primer by company (33 pieces in total)
- Repeat PCR for many times, recovery them and then get the complete gene product
- Sub cloning vector and sequenced
- Try to express by E.coli and analyze the effect of this protein
- Express by lactic acid bacteria
July to Sept, 2014
- Discussion about many possible paths include MC-LR
- Try cloning Pseudomonas natural promoter.(non-coding sequences in Mlr enzyme series )
- Synthesis by pieces then got complete product.
- The gene transformed into E.coli and verify its functions.
- Transform this gene by many ways into lactic acid bacteria and verify the function of promoter.
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