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Team:Paris Saclay/Notebook/July/17
From 2014.igem.org
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5uL of antibiotic in each tube. | 5uL of antibiotic in each tube. | ||
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| + | ===3 - PCR and Electrophoresis=== | ||
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| + | ''by Arnaud & Mathias'' | ||
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| + | PCR targeting with oligos IPS70 and IPS71. | ||
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| + | #IPS70 and IPS71 (1) | ||
| + | #IPS70 and IPS71 (2) | ||
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| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 07:55, 18 July 2014
Contents |
Thursday 17th July
Lab work
1 - Preparation of supercompetent cells
By Mathias & Romain
Strains used: MG1655 (x2), MG1655Z1 (x2).
2 - Bacterial Culture
by Fabio and Maher
Strains used: J23119 from the concentrated colony made the 16th July
5uL of antibiotic in each tube.
3 - PCR and Electrophoresis
by Arnaud & Mathias
PCR targeting with oligos IPS70 and IPS71.
- IPS70 and IPS71 (1)
- IPS70 and IPS71 (2)
