Team:Cooper Union/Notebook/Biohack May

From 2014.igem.org

(Difference between revisions)
 
Line 110: Line 110:
<h3>5/28/14</h3>
<h3>5/28/14</h3>
<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
-
 
Line 137: Line 136:
             <span>Projects</span>
             <span>Projects</span>
         </a>
         </a>
-
         <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" >
+
         <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
-
             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" />
+
             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
             <span>Social</span>
             <span>Social</span>
         </a>
         </a>

Latest revision as of 00:45, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


5/19/14

  • Calibrated Nanodrop.
  • Nanodropped all of our samples. Very low concentration of DNA.
  • Ordered primers to PCR our g-blocks.

5/22/14

  • Struck out plates with pACYC184 with spectinomycin to grow it.
  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII/EcoRI.

5/23/14

  • Ran gel of digests from 5/22/14. Everything as it should be.
  • Purified the digested plasmids.
  • Nanodropped plasmids; results:
    Single digest I: 52.7ng/μL
    Single digest II: 64.5ng/μL
    Double digest I: 26.3ng/μL
    Double digest II: 41.9ng/μL
  • Phosphatase treated the four digested plasmids.

5/27/14

  • Purified phosphatase treated plasmids from 5/23/14.
  • Nanodropped the purified plasmids; results:
    Single digest I: 43.4ng/μL
    Single digest II: 53.6ng/μL
    Double digest I: 43.7ng/μL
    Double digest II: 16.5ng/μL

5/28/14



Projects Social Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_May"