Team:Cambridge-JIC/Notebook/CL 2014 7 17

From 2014.igem.org

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<p><strong>Salil</strong><br>Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.
<p><strong>Salil</strong><br>Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.
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edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...
edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...

Revision as of 00:09, 18 July 2014

Home Team Official Team Profile TimeLine Project Outreach Marchantia Informatics Parts Modeling Notebook Protocols Safety Dry Work Technology Attributions

Hugh
Work on the wiki; listened to Gos Micklem discuss good lab technique

Salil
Played around with Fernan's construct - getting nowhere. Realised there may be a way in. Collected DNA from Bernardo and loaded CC E-coli with it, for Guy to finish off a bit later. Intent upon ordering some primers tomorrow.

edit, 0106: just realised it may be possible to hack together two plasmids to build an almost full circuit.. excited to pitch plan to someone (a bit later today) with more experience in restriction/ligation cloning...