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Team:Peking/Parts
From 2014.igem.org
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<div id="fpartwcg1" class="partswcg"> | <div id="fpartwcg1" class="partswcg"> | ||
<p><b>INPNC-Linker (rTEV)-MVN-mRFP</b></p> | <p><b>INPNC-Linker (rTEV)-MVN-mRFP</b></p> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378005">Main Page-BBa_K1378005</a> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378005" target="_blank">Main Page-BBa_K1378005</a> |
<p>BBa_K1378005 is a composite part coding INPNC-Linker (rTEV)-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.</p> | <p>BBa_K1378005 is a composite part coding INPNC-Linker (rTEV)-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.</p> | ||
| - | <p><a href="https://2014.igem.org/Team:Peking/KillingImprovements">Visit Wiki</a> | + | <p><a href="https://2014.igem.org/Team:Peking/KillingImprovements" target="_blank">Visit Wiki</a> |
</div> | </div> | ||
<div id="fpartwcg2" class="partswcg"> | <div id="fpartwcg2" class="partswcg"> | ||
<p><b>MlrA</b></p> | <p><b>MlrA</b></p> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378001">Main Page-BBa_K1378001</a> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378001" target="_blank">Main Page-BBa_K1378001</a> |
<p>MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in <i>E.coli</i> and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to protein phosphatase 1.</p> | <p>MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in <i>E.coli</i> and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to protein phosphatase 1.</p> | ||
| - | <p><a href="https://2014.igem.org/Team:Peking/Degradation">Visit Wiki</a> | + | <p><a href="https://2014.igem.org/Team:Peking/Degradation" target="_blank">Visit Wiki</a> |
</div> | </div> | ||
<div id="fpartwcg3" class="partswcg"> | <div id="fpartwcg3" class="partswcg"> | ||
<p><b>INPNC-MVN & GFP</b></p> | <p><b>INPNC-MVN & GFP</b></p> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378007">Main Page-BBa_K1378007</a> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378007" target="_blank">Main Page-BBa_K1378007</a> |
<p>BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. <i>E.coli</i> carrying this plasmid will display MVN on the cell surface and bind to <i>M. aeruginosa</i> as well as expression GFP, thus the binding phenomenon can be easily observed.</p> | <p>BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. <i>E.coli</i> carrying this plasmid will display MVN on the cell surface and bind to <i>M. aeruginosa</i> as well as expression GFP, thus the binding phenomenon can be easily observed.</p> | ||
| - | <p><a href="https://2014.igem.org/Team:Peking/KillingImprovements">Visit Wiki</a> | + | <p><a href="https://2014.igem.org/Team:Peking/KillingImprovements" target="_blank">Visit Wiki</a> |
</div> | </div> | ||
| Line 53: | Line 53: | ||
<h2 id="parts02" style="margin-top:20px;">Part List</h2> | <h2 id="parts02" style="margin-top:20px;">Part List</h2> | ||
<h3>Parts for Killing Improvement</h3> | <h3>Parts for Killing Improvement</h3> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378003">BBa_K1378003</a> is the coding sequence of lectin Microvirin.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378003" target="_blank">BBa_K1378003</a> is the coding sequence of lectin Microvirin.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378005">BBa_K1378005</a> is the coding sequence of INPNC-MVN-mRFP fusion protein.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378005" target="_blank">BBa_K1378005</a> is the coding sequence of INPNC-MVN-mRFP fusion protein.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378007">BBa_K1378007</a> is a composite part of GFP and INPNC-MVN fusion protein.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378007" target="_blank">BBa_K1378007</a> is a composite part of GFP and INPNC-MVN fusion protein.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378009">BBa_K1378009</a> is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378009" target="_blank">BBa_K1378009</a> is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.</p> |
<br/><br/> | <br/><br/> | ||
<h3>Parts for Degradation</h3> | <h3>Parts for Degradation</h3> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378001">BBa_K1378001</a> is protease MlrA which can specifically cleavage Microcystin(MC).</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378001" target="_blank">BBa_K1378001</a> is protease MlrA which can specifically cleavage Microcystin(MC).</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378022">BBa_K1378022</a> is the coding sequence of MlrA fused with PelB secretion tag.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378022" target="_blank">BBa_K1378022</a> is the coding sequence of MlrA fused with PelB secretion tag.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378023">BBa_K1378023</a> is the coding sequence of MlrA fused with TorA secretion tag.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378023" target="_blank">BBa_K1378023</a> is the coding sequence of MlrA fused with TorA secretion tag.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378024">BBa_K1378024</a> is the coding sequence of MlrA with terminator.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378024" target="_blank">BBa_K1378024</a> is the coding sequence of MlrA with terminator.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378025">BBa_K1378025</a> is the coding sequence of PelB-MlrA fusion protein with terminator.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378025" target="_blank">BBa_K1378025</a> is the coding sequence of PelB-MlrA fusion protein with terminator.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378026">BBa_K1378026</a> is the coding sequence of TorA-MlrA fusion protein with terminator.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378026" target="_blank">BBa_K1378026</a> is the coding sequence of TorA-MlrA fusion protein with terminator.</p> |
<br/><br/> | <br/><br/> | ||
<h3>Parts for Suicide</h3> | <h3>Parts for Suicide</h3> | ||
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378031">BBa_K1378031</a> is the coding sequence for holin.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378031" target="_blank">BBa_K1378031</a> is the coding sequence for holin.</p> |
| - | <p><a href="http://partsregistry.org/Part:BBa_K1378032">BBa_K1378032</a> is the coding sequence for endolysin.</p> | + | <p><a href="http://partsregistry.org/Part:BBa_K1378032" target="_blank">BBa_K1378032</a> is the coding sequence for endolysin.</p> |
Revision as of 00:29, 18 October 2014
Favorite Parts
INPNC-Linker (rTEV)-MVN-mRFP
BBa_K1378005 is a composite part coding INPNC-Linker (rTEV)-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.
MlrA
MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to protein phosphatase 1.
INPNC-MVN & GFP
BBa_K1378007 is a composite part coding INPNC-MVN fusion protein and GFP. E.coli carrying this plasmid will display MVN on the cell surface and bind to M. aeruginosa as well as expression GFP, thus the binding phenomenon can be easily observed.
Part List
Parts for Killing Improvement
BBa_K1378003 is the coding sequence of lectin Microvirin.
BBa_K1378005 is the coding sequence of INPNC-MVN-mRFP fusion protein.
BBa_K1378007 is a composite part of GFP and INPNC-MVN fusion protein.
BBa_K1378009 is a composite part of GFP and INPNC-MVN fusion protein with a linker between INPUC and MVN which can be cleavaged by rTEV.
Parts for Degradation
BBa_K1378001 is protease MlrA which can specifically cleavage Microcystin(MC).
BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.
BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.
BBa_K1378024 is the coding sequence of MlrA with terminator.
BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.
BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.
Parts for Suicide
BBa_K1378031 is the coding sequence for holin.
BBa_K1378032 is the coding sequence for endolysin.
