Team:Arizona State/policypractices

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         <li><a href="https://2014.igem.org/Team:Arizona_State/policypractices">policy and practices</a></li></ul></li>
         <li><a href="https://2014.igem.org/Team:Arizona_State/policypractices">policy and practices</a></li></ul></li>
   <li><a href="https://2014.igem.org/Team:Arizona_State/parts">parts </a></li>
   <li><a href="https://2014.igem.org/Team:Arizona_State/parts">parts </a></li>
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  <li><a href="https://2014.igem.org/Team:Arizona_State/result">results </a></li>
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   <li><a href="https://2014.igem.org/Team:Arizona_State/notebook">notebook </a></li>
   <li><a href="https://2014.igem.org/Team:Arizona_State/notebook">safety </a></li>
   <li><a href="https://2014.igem.org/Team:Arizona_State/notebook">safety </a></li>

Revision as of 00:21, 18 October 2014


 

 

Policy


 

Policy and Practices

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Policy and Practices

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Practices


 

Resting Cell Assay

To test the productivity of different plasmids, resting cell assays were run. All assays were run in a shaker incubator at 32 degrees C and 200 RPM. A phosphate buffered solution was used to suspend the growth of the cultures.

 

Quantification

A GC with a DB5 column was used to quantify data from all assays. For testing ethanol production, the method used had an initial temp. of 60 degrees C, and ramped by 10 degrees C until it reached 250 degrees C, which was then held for 3 minutes.