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Team:Freiburg/Content/Notebook
From 2014.igem.org
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Revision as of 00:10, 18 October 2014
p14rz_002
- PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
- Digest: pKM006, EcoRI + NotI; PCR-product
- Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7
PCR
Testdigest
p14rz_003
- PCR 3
- Digest 3, 4
- Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8
PCR
Digest
Testdigest
p14rz_004
- PCR 21,22
- Digest -
- Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2
PCR
PCR
Testdigest
p14rz_005
- PCR 4,5
- Digest 5
- Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3
PCR
Digest
p14rz_006
- PCR 17,18
- Digest 5
- Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4
PCR
Digest
Testdigest
p14rz_007
- PCR 37,38
- Digest 21
- Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7
PCR
Digest
Testdigest
p14rz_008
- PCR blue light receptor; Template: pQXCIN-SLC7A1
- Digest pKM006 EcoRI, NotI
- Testdigest with SmaI in Cutsmart, expected bands: 5,7
PCR
Testdigest
Testdigest
p14rz_009
- PCR 39,40
- Digest 5
- Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7
PCR
Digest
Testdigest
p14rz_010
- PCR 23,24
- Digest 12
- Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56
PCR
PCR
Testdigest
p14ls_002
- PCR 7,8
- Digest -
- Testdigest with PstI in Cutsmart, expected bands: 4,1;0,56
PCR
Digest
Testdigest
p14ls_003
- PCR 7,9,10
- Digest -
- Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4
PCR
Digest
Testdigest
p14vv_001
- PCR Primers vv003,vv004; Template: pMIG
- Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
- Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6
Digest
Digest
Testdigest
p14vv_002
- PCR -
- Digest 8,11
- Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4
PCR
Digest
Testdigest
p14vv_003
- PCR pMIG with primer vv003+vv004-
- Digest xx
- Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5
PCR
Digest
Testdigest
p14vv_006
- PCR 26
- Digest 13
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6
PCR
Digest
Testdigest
p14vv_007
- PCR 27
- Digest 14
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66
PCR
Digest
Testdigest
p14vv_008
- PCR 43
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75
PCR
Digest
Testdigest
p14vv_009
- PCR 44
- Digest 22
- Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75
PCR
Digest
Testdigest
p14vv_010
- PCR 45
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2
PCR
Digest
Testdigest
p14vv_012
- PCR 46
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4
PCR
Digest
Testdigest
p14vv_013
- PCR 47,48,49
- Digest 22
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2
PCR
Digest
Testdigest
p14vv_014
- PCR 50,51
- Digest 22
- Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66
PCR
Digest
Testdigest
p14zl_003
- PCR xx
- Digest xx
- Testdigest with NruI+MluI in Cutsmart, expected bands: 4,9;3,3;2,7;0,7
- Testdigest with NgoMIV in Cutsmart, expected bands: 4,7;4,1;2,8
PCR
Digest
Testdigest
p14zl_010
- PCR 1,2
- Digest 1
- Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1
PCR
Digest
Testdigest
p14zl_011
- PCR 19,20
- Digest 1
- Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43
PCR
Digest
Testdigest
p14zl_016
- PCR 52,53
- Digest 22
- Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7
PCR
Digest
Testdigest
p14zl_017
- PCR 64,65
- Digest 24
- testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9
PCR_digest
PCR
Testdigest
p14zl_018
- PCR 55,56
- Digest 24
- testdigest with NgoMIV in Cutsmart, expected bands: 4,7;2,5
PCR
Digest
Testdigest
p14zl_019
- PCR 57,58
- Digest 24
- testdigest with PstI in Cutsmart, expected bands: 5,8;2,6;1,1;0,2
PCR
Digest
Testdigest
p14zl_020
- PCR 59,60
- Digest 24
- testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8
PCR
Digest
Testdigest
p14zl_023
- PCR 68,69
- Digest 22
- testdigest with EcoRV in Cutsmart, expected bands: 3,4;2,5
PCR
Digest
Testdigest
