Team:Imperial/Lab-book

From 2014.igem.org

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                         <p>LINKS</p>
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                         <p>Precise cloning reaction set-ups are detailed <a href="https://static.igem.org/mediawiki/2014/a/af/Interlab-study-cloning-lab-work-record.xls">here</a></p>
                          
                          
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Revision as of 00:03, 18 October 2014

Imperial iGEM 2014

Lab-books

Introduction

We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.

General Mixed LabWork

This document contains experimental details for some of the following parts of the project

  • Bulk cellulose growth and processing techniques
  • Coculture experiments
  • G. xylinus isolation and culturing
  • Initial cloning work for Acs operon
  • Preliminary work for isolating curli genes

Primer Inventory

Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our inventory

Functionalisation cloning

This lab-book is a break-away from the mixed lab-book and contains details of cloning the constructs used in the functionalisation part of our project.

This spreadsheet also details specific digestions and ligation set-ups for these constructs.

Interlab study cloning

Precise cloning reaction set-ups are detailed here

Lab-book 1

LINKS