Team:Groningen/Template/MODULE/Notebook/secretion/week7

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8 – 12 September
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September 8 - September 12
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<b>Goal:</b> analysis of the possible made Biobricks.
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Analysis of the constructed Biobricks
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Because of the very low amount of gblocks given, a decision was made to do a PCR directly on the product itself, therefor multiplying it exponentially.
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<div class="item">PCR was performed directly on the ssUSP45DspB product</div>
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<div class="item">The plamids containing  the ssUSP45aiiA was isolated our of <i>E. coli</i> DH5α and checked on gel, which showed some positive results for the contructs</div>
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<div class="item">New primer were designed, to give it another try</div>
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<div class="item">the first BioBricks were send for sequencing </div>
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All the O/N cultures were mini-prepped. The possible plasmids were cut with EcoRI and SpeI. After running on gel, the result showed us that 20 of the 40 clones contained all the Biobricks, though with a low yield of plasmids.
 
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A new strategy of assembling has been prepared and primers for this strategy have been made.
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All the Biobricks were send for sequencing.
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Latest revision as of 23:59, 17 October 2014

September 8 - September 12
 
Analysis of the constructed Biobricks
 
PCR was performed directly on the ssUSP45DspB product
The plamids containing the ssUSP45aiiA was isolated our of E. coli DH5α and checked on gel, which showed some positive results for the contructs
New primer were designed, to give it another try
the first BioBricks were send for sequencing
 
 
 
 
 

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