Team:Penn/Protocol
From 2014.igem.org
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<h3>E.Coli Specific Protocols</h3> | <h3>E.Coli Specific Protocols</h3> | ||
- | <p style = "text-align: left; text-indent:0px"><a href="">Miniprep</a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/7e/Mini_Prep.pdf">Miniprep</a></p> |
- | <p style = "text-align: left; text-indent:0px"><a href="">Cloning PCR </a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/0/0c/Cloning_PCR_Procedures.pdf">Cloning PCR </a></p> |
- | <p style = "text-align: left; text-indent:0px"><a href="">Colony PCR</a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/1/15/Protocol_Colony.pdf">Colony PCR</a></p> |
- | <p style = "text-align: left; text-indent:0px"><a href="">Gel Extraction</a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c6/Gel_Extraction.pdf">Gel Extraction</a></p> |
- | <p style = "text-align: left; text-indent:0px"><a href="">PCR Cleanup</a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup</a></p> |
- | <p style = "text-align: left; text-indent:0px"><a href=" | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/94/Protocol.pdf">Ligation</a></p> |
- | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup </a></p> | |
- | <p style = "text-align: left; text-indent:0px"><a href=" | + | |
- | + | ||
<h3>AMB-1 Specific Protocols</h3> | <h3>AMB-1 Specific Protocols</h3> |
Revision as of 23:56, 17 October 2014
Protocols
E.Coli Specific Protocols
AMB-1 Specific Protocols
Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.
Click here to view the Spec Sheet!Inoculation of Aerobic Cultures
Penn iGEM Transformation Protocol
Materials and Methods
Determining Magnetic Properties of AMB-1
Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures