"
Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0032 mRFP Assay
From 2014.igem.org
(Difference between revisions)
Kakurehana (Talk | contribs) |
|||
| Line 82: | Line 82: | ||
</ul> | </ul> | ||
<ul> | <ul> | ||
| - | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High | + | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High School</a></li> |
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li> | ||
Revision as of 23:52, 17 October 2014
Notebook
Lab Documents
140910 asB0034 & B0032 mRFP Assay
-
Start
-
Transformation
Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0032-mRFP-dt on pSB6A1 on ice 30min each of 2.5µL DNA to DH5α 42℃ 30sec on ice 2min -
Colony PCR
Ptet-B0032-mRFP-dT on pSB6A1 EcoI_XbaI_F Tm:70.9℃ rbs_SpeI_R Tm:59.2℃ -
Preculture
Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0032-mRFP-dT(pSB6A1) LB 2 mL for 8hours -
Culture
M9ZB 2 mL IPTG 20 µ or not for 9 hours, 11 hours, 13hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
