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Team:Aberdeen Scotland/Project/Device
From 2014.igem.org
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| - | <p>In order to be able to test a patient in the field, the assay needs to be analysed. As the assay results in expressed in GFP (Green Fluorescent Protein) production, it is going to be very hard to make any meaningful measurements by hand. This is why our team has concentrated on designing a portable device that would aid the medical staff.</p> | + | <p>In order to be able to test a patient in the field, the assay needs to be analysed. As the assay results in expressed in GFP (Green Fluorescent Protein) |
| - | <p>The operation of the device in question relies on several fundamental principles - the discrete nature of the activation and emission wavelengths for GFP, the filtering of UV light and the sensitivity range of LDRs (Light Dependent Resistors). Essentially, the device is going to measure the intensity of fluorescent light due to GFP illumination with UV light, in order to determine the amount (concentration) of GFP in the medium.</p> | + | production, it is going to be very hard to make any meaningful measurements by hand. This is why our team has concentrated on designing a portable device that would |
| + | aid the medical staff.</p> | ||
| + | <p>The operation of the device in question relies on several fundamental principles - the discrete nature of the activation and emission wavelengths for GFP, the | ||
| + | filtering of UV light and the sensitivity range of LDRs (Light Dependent Resistors). Essentially, the device is going to measure the intensity of fluorescent light | ||
| + | due to GFP illumination with UV light, in order to determine the amount (concentration) of GFP in the medium.</p> | ||
<h3>The Measurement Chamber</h3> | <h3>The Measurement Chamber</h3> | ||
| - | <p>After the assay protocol has been finished, the cuvette is to be placed in the measurement chamber of the device. There a UV LED (Light Emitting Diode) with peak wavelength at 395 nm (the activation wavelength for GFP) | + | <p>After the assay protocol has been finished, the cuvette is to be placed in the measurement chamber of the device. There a UV LED (Light Emitting Diode) with peak |
| + | wavelength at 395 nm (the activation wavelength for GFP) shines through the cuvette and illuminates the GFP molecules in the medium. On the other side of the | ||
| + | cuvette, after a UV filter and a Green (green pass-through) filter, the light intensity is measured by means of an LDR (Light Dependent Resistor).</p> | ||
<img src="https://static.igem.org/mediawiki/2014/6/62/Chamber.png"> | <img src="https://static.igem.org/mediawiki/2014/6/62/Chamber.png"> | ||
<h3>Control Circuit</h3> | <h3>Control Circuit</h3> | ||
| - | <p>The LDR is | + | <p>The LDR is connected to an Arduino, that manages and supplies power to the measuring chamber and the measuring process. In order to improve |
| + | on the noisy direct-measurement values, the Arduino communicates with a RaspberryPi, that uses Wolfram Mathematica 10 (incl) software for noise reduction and data | ||
| + | analysis. In certain cases the RaspberryPi will be able to react to incoming data by adjusting the measurement procedure employed by the Arduino through the control | ||
| + | API.</p> | ||
<img src="https://static.igem.org/mediawiki/2014/1/18/Circuit.png"> | <img src="https://static.igem.org/mediawiki/2014/1/18/Circuit.png"> | ||
<h3>Portability</h3> | <h3>Portability</h3> | ||
| - | <p>The final milestone for the device is going to be portability. With the low-power nature of all the components in question, | + | <p>The final milestone for the device is going to be portability. With the low-power nature of all the components in question, the device is able |
| + | to operate from a small USB battery pack. The battery is charged in the mains or through a solar-panel charger. The market for such solar chargers is | ||
| + | growing rapidly and they can be found on very affordable prices with good reliability.</p> | ||
<h3>Price</h3> | <h3>Price</h3> | ||
<ul> | <ul> | ||
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<ul> | <ul> | ||
<li>UV LED (395nm)<a href="http://uk.rs-online.com/web/p/uv-leds/7134894/"><sup>1</sup></a> <span style="float:right">£1.07</span></li> | <li>UV LED (395nm)<a href="http://uk.rs-online.com/web/p/uv-leds/7134894/"><sup>1</sup></a> <span style="float:right">£1.07</span></li> | ||
| - | <li>UV Filter<a href="http://www.epakelectronics.com/uv_filter_materials.htm"><sup>2</sup></a> <span style="float:right">£10</span></li> | + | <li>UV Filter<a href="http://www.epakelectronics.com/uv_filter_materials.htm"><sup>2</sup></a> <span style="float:right">£10.00</span></li> |
<li> | <li> | ||
Green Filter<a href="http://www.pnta.com/lighting/gels/lee/lee-filters-cl139-cool-led-primary-green/"><sup>3</sup></a> | Green Filter<a href="http://www.pnta.com/lighting/gels/lee/lee-filters-cl139-cool-led-primary-green/"><sup>3</sup></a> | ||
<a href="http://www.leefilters.com/lighting/colour-details.html#CL139&filter=ld"><sup>4</sup></a> <span style="float:right">£4.26</span> | <a href="http://www.leefilters.com/lighting/colour-details.html#CL139&filter=ld"><sup>4</sup></a> <span style="float:right">£4.26</span> | ||
</li> | </li> | ||
| - | <li>LDR (Light Dependent Resistor)<a href="http://uk.rs-online.com/web/p/ldr-light-dependent-resistors/2341050/"><sup>5</sup></a> <span style="float:right">£0.91</span></li> | + | <li>LDR (Light Dependent Resistor) |
| + | <a href="http://uk.rs-online.com/web/p/ldr-light-dependent-resistors/2341050/"><sup>5</sup></a> <span style="float:right">£0.91</span></li> | ||
<li>Arduino UNO<a href="http://r.ebay.com/cDXfED"><sup>6</sup></a> <span style="float:right">£6.99</span></li> | <li>Arduino UNO<a href="http://r.ebay.com/cDXfED"><sup>6</sup></a> <span style="float:right">£6.99</span></li> | ||
<li>Raspberry Pi<a href="http://goo.gl/lRVsOO"><sup>7</sup></a> <span style="float:right">£25.80</span></li> | <li>Raspberry Pi<a href="http://goo.gl/lRVsOO"><sup>7</sup></a> <span style="float:right">£25.80</span></li> | ||
| - | <li>Raspberry Pi Starter Kit<a href="http://goo.gl/InsQjb"><sup>8</sup></a> <span style="float:right">£15</span></li> | + | <li>Raspberry Pi Starter Kit<a href="http://goo.gl/InsQjb"><sup>8</sup></a> <span style="float:right">£15.00</span></li> |
</ul> | </ul> | ||
</li> | </li> | ||
Revision as of 23:51, 17 October 2014
Portable Fluorescence Detector
The measurement device for automatically and efficiently analysing the test results on the go.
In order to be able to test a patient in the field, the assay needs to be analysed. As the assay results in expressed in GFP (Green Fluorescent Protein) production, it is going to be very hard to make any meaningful measurements by hand. This is why our team has concentrated on designing a portable device that would aid the medical staff.
The operation of the device in question relies on several fundamental principles - the discrete nature of the activation and emission wavelengths for GFP, the filtering of UV light and the sensitivity range of LDRs (Light Dependent Resistors). Essentially, the device is going to measure the intensity of fluorescent light due to GFP illumination with UV light, in order to determine the amount (concentration) of GFP in the medium.
The Measurement Chamber
After the assay protocol has been finished, the cuvette is to be placed in the measurement chamber of the device. There a UV LED (Light Emitting Diode) with peak wavelength at 395 nm (the activation wavelength for GFP) shines through the cuvette and illuminates the GFP molecules in the medium. On the other side of the cuvette, after a UV filter and a Green (green pass-through) filter, the light intensity is measured by means of an LDR (Light Dependent Resistor).
Control Circuit
The LDR is connected to an Arduino, that manages and supplies power to the measuring chamber and the measuring process. In order to improve on the noisy direct-measurement values, the Arduino communicates with a RaspberryPi, that uses Wolfram Mathematica 10 (incl) software for noise reduction and data analysis. In certain cases the RaspberryPi will be able to react to incoming data by adjusting the measurement procedure employed by the Arduino through the control API.
Portability
The final milestone for the device is going to be portability. With the low-power nature of all the components in question, the device is able to operate from a small USB battery pack. The battery is charged in the mains or through a solar-panel charger. The market for such solar chargers is growing rapidly and they can be found on very affordable prices with good reliability.
