Team:NTU Taida/M3

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In order to efficiently select the adequate promoters, Ribosome binding Sites(RBSs) and inducer concentrations with desired Input/output characteristics, we use the promoter- RBS libraries and the mathematical dynamic model refer in [7] to describe the protein expression in a more comprehensive way.</p>
In order to efficiently select the adequate promoters, Ribosome binding Sites(RBSs) and inducer concentrations with desired Input/output characteristics, we use the promoter- RBS libraries and the mathematical dynamic model refer in [7] to describe the protein expression in a more comprehensive way.</p>
The figure below illustrates overview of the genetic circuit. Crucial elements and their relations are cited in the figure.
The figure below illustrates overview of the genetic circuit. Crucial elements and their relations are cited in the figure.
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<center><img src="images/h6.jpg", height="500" > </p></center>
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<center><img src="https://static.igem.org/mediawiki/2014/e/e7/NTU_Taida_H6.jpg", height="500" > </p></center>
<center><u>Fig 4 Representation of the genetic circuit. The output signal is measured by GFP reporter.</u></center>
<center><u>Fig 4 Representation of the genetic circuit. The output signal is measured by GFP reporter.</u></center>
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<center><img src="images/g7.jpg", height="500" > </p></center>
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<center><img src="https://static.igem.org/mediawiki/2014/a/a3/NTU_Taida_G7.jpg", height="500" > </p></center>
<center><u>Fig 4(cont’)</u></center>
<center><u>Fig 4(cont’)</u></center>
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<div class="boxK"style="position:relative; top:100px;padding:200px;padding-top:25px;padding-bottom:25px">
Mathematical dynamic model with identified kinetic strength of promoter-RBS is described as follow :</p>
Mathematical dynamic model with identified kinetic strength of promoter-RBS is described as follow :</p>
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<center><img src="images/h9.jpg", height="400" > </p></center>
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<center><img src="https://static.igem.org/mediawiki/2014/2/22/NTU_Taida_H9.jpg", height="400" > </p></center>
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Revision as of 23:47, 17 October 2014

NTU-Taida

Model 2:

The protein production is regulated by the concentrations of inducer, repressor ,activator and the different combinations of promoters with RBSs.However, in the past the strength of a promoter and an RBS, which are the main components of transcription and translation were only defined relatively to other promoter-RBS component. It lacks a standard way to quantify the strength of promoter-RBS component as standard Biobrick.

In order to efficiently select the adequate promoters, Ribosome binding Sites(RBSs) and inducer concentrations with desired Input/output characteristics, we use the promoter- RBS libraries and the mathematical dynamic model refer in [7] to describe the protein expression in a more comprehensive way.

The figure below illustrates overview of the genetic circuit. Crucial elements and their relations are cited in the figure.

Fig 4 Representation of the genetic circuit. The output signal is measured by GFP reporter.

Fig 4(cont’)
Mathematical dynamic model with identified kinetic strength of promoter-RBS is described as follow :

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