Team:NTU Taida/M1
From 2014.igem.org
(Difference between revisions)
Line 205: | Line 205: | ||
<b>1. Prediction of fundamentals of exfoliation circuit</p></b> | <b>1. Prediction of fundamentals of exfoliation circuit</p></b> | ||
In our circuit design of exfoliation, one of core concept is using lambda repressor(CI) to regulate the gene expression of keratinase. We have written a script in matlab to simulate this system repression according to [9]. In our simulation, we assume that when pfdba promoter is working, it will work as constitutive promoter,and the mathematical descriptions of the model are:</p> | In our circuit design of exfoliation, one of core concept is using lambda repressor(CI) to regulate the gene expression of keratinase. We have written a script in matlab to simulate this system repression according to [9]. In our simulation, we assume that when pfdba promoter is working, it will work as constitutive promoter,and the mathematical descriptions of the model are:</p> | ||
- | <center><img src=" | + | <center><img src="https://static.igem.org/mediawiki/2014/5/5f/NTU_Taida_H1.jpg" > </p></center> |
The model consists of basal expression(α0Y),protein synthesis(αC,αY),repressor binding(βC),protein decay(γC,γY) and repressor cooperativity(ηC) for CI(C) and keratinase(Y).Following figure 1 shows the result of simulation.</p> | The model consists of basal expression(α0Y),protein synthesis(αC,αY),repressor binding(βC),protein decay(γC,γY) and repressor cooperativity(ηC) for CI(C) and keratinase(Y).Following figure 1 shows the result of simulation.</p> | ||
- | <center><img src=" | + | <center><img src="https://static.igem.org/mediawiki/2014/0/0c/NTU_Taida_H2.jpg" > </p></center> |
<center><u>Fig 1 simulation prediction of exfoliation circuit</u></center> | <center><u>Fig 1 simulation prediction of exfoliation circuit</u></center> | ||
</div> | </div> |
Revision as of 23:40, 17 October 2014