Team:Penn/Protocol

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Revision as of 23:31, 17 October 2014

University of Pennsylvania iGEM

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Protocols

E.Coli Specific Protocols

Restriction Enzyme Digest

Ligation

Transformation

Innoculation

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Click here to view the Spec Sheet!

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques

@@ Line 59: / Line 59: @@
 <h3>E.Coli Specific Protocols</h3>
-<p><a href="">Miniprep</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Miniprep</a></p>
-<p><a href="">Cloning PCR </a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Cloning PCR </a></p>
-<p><a href="">Colony PCR</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Colony PCR</a></p>
-<p><a href="">Gel Extraction</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Gel Extraction</a></p>
-<p><a href="">PCR Cleanup</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">PCR Cleanup</a></p>
-<p><a href="">Restriction Enzyme Digest</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Restriction Enzyme Digest</a></p>
-<p><a href="">Ligation</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Ligation</a></p>
-<p><a href="">Transformation </a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Transformation </a></p>
-<p><a href="">Innoculation</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="">Innoculation</a></p>
 <h3>AMB-1 Specific Protocols</h3>