Team:Penn/Protocol

From 2014.igem.org

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<div style = "text-align: center; font-size: 24px;">Protocols</div></br>
<div style = "text-align: center; font-size: 24px;">Protocols</div></br>
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<h3>E.Coli Specific Protocols</h3>
Miniprep
Miniprep
Cloning PCR  
Cloning PCR  
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<h3>AMB-1 Specific Protocols</h3>
<h3>AMB-1 Specific Protocols</h3>
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<p> Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
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<<p style = "text-align: left; text-indent:0px"> Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
<a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br>
<a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br>

Revision as of 23:28, 17 October 2014

University of Pennsylvania iGEM

Protocols

E.Coli Specific Protocols

Miniprep Cloning PCR Colony PCR Gel Extraction PCR Cleanup Restriction Enzyme Digest Ligation Transformation Inoculation E.Coli Specific Protocols

Gel Extraction

AMB-1 Specific Protocols

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Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Click here to view the Spec Sheet!

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques

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