Team:British Columbia/Notebook/Protocol/Biobrickdigest

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Revision as of 23:26, 17 October 2014

> 2014 UBC iGEM



BioBrick Restriction Digest

Supplies:

  • dsH2O
  • 10x buffer (1,2,3,4)
  • 100x BSA
  • Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)
  • DNA

Steps:

  1. Make a master mix with everything except the DNA. Keep everything on ice.
    2. https://static.igem.org/mediawiki/2013/9/92/DigestA.JPG *depending on DNA concentration*
  2. Pipet aliquots to each sample tube and add DNA.
  3. Incubate at 37°C for 2-3h or let digest over night at room temperature.

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