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Team:British Columbia/Notebook/Protocol
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Instruction for colony PCR and regular PCR | Instruction for colony PCR and regular PCR | ||
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| - | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocol/gelverification</a> </font> </b> | + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocol/gelverification"> Gel Verification |
| + | </a> </font> </b> | ||
</li> | </li> | ||
Make a gel and conduct gel elecrophoresis | Make a gel and conduct gel elecrophoresis | ||
Latest revision as of 23:26, 17 October 2014
Protocols
- PCR Instruction for colony PCR and regular PCR
- Gel Verification Make a gel and conduct gel elecrophoresis
- BioBrick Restriction Digest Restriction digest with BioBrick plasmid an inserts
- BioBrick Ligation Ligate Biobrick insert to vector
- Making Plates Make LB plates with antibiotics
- Liquid media Make liquid LB media
- Competent cells Preparation of chemically competent cells
- Transformation Transformation of plasmi DNA into chemically competent cells
- CRISPR Repeat-Spacer Array Assembly Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
- Assembling Parts from Oligonucleotides Assemble short parts from annealed oligonucleotides
- (Multi) Site Directed Mutagenesis Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
- Oligonucleotide Phosphorylation Phosphorylate oligonucleotides prior to ligation
- Electrocompetent Cell Production Make electrocompetent cells
- Make Combinatorial Part Libraries Make combinatorial part libraries from compatible parts
