Team:Northwestern/CFPS

From 2014.igem.org

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Latest revision as of 23:19, 17 October 2014

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Cell-Free Protein Synthesis

20140723 Testing our lysates
  • data from BL21 (E.Coli) lysates
  • out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
  • the plasmid used was a construct of superfolder GFP from the Jewett Lab
  • conclusion:our lysates are functional
red: control, blue: BL21 lysate
20140801 Making our own mastermix (iMM)!
  • RM: Rey's mastermix
  • iMM: we made our own according to Rey's recipe
  • looks like our mastermix works fine
  • conclusion: we will use our own mastermix from now on
  • the second part: we made s. rimosus lysates
  • we used a sfgfp construct from the Jewett lab
  • the lysates seem to express some gfp!
20140915 All of our lysates
  • RBS B0034
  • Tried all of our lysates
  • green fluorescence indicates mRNA levels of transcription
  • red fluorescence indicates protein levels of translation
  • conclusion: the data is most likely statistically insignificant
20141014 Cell-Free Protein Synthesis Assay with All of our Lysates
  • We used RBS No. 5 (B0034) with RFP-Spinach Aptamer
  • Displayed here is the first read
  • The kinetic portion of the plate reader did not record data in all of our wells
  • We will continue doing CFPS experiments
  • conclusion: we need more time! Looks like fluorophores were incorrectly added

Retrieved from "http://2014.igem.org/Team:Northwestern/CFPS"