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| - | <div style="background-color:white; border-bottom-left-radius:10px;border-bottom-right-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:100px;">
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| - | <br>
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| - | <font style=“font-weight: 600;”>Wetlab Results:</font> <br><br>
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| - | For the bioremediation aspect of DCMation, we have managed to:<br><br>
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| - | 1. purify the pUNI-ABTUNJK and pSB1C3-ABTUNJK plasmids, which encode the microcompartments<br>
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| - | 2. verify expression of pUNI-ABTUNJK plasmids in E. coli using Western blotting<br>
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| - | 3. insert the ABTUNJK vector into pSRKGm using Gibson assembly, for expression in P. putida <br>
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| - | 4. insert the dcmA gene into the pCM66 backbone using Gibson assembly, since pCM66 is suitable for expression in Methylobacterium extorquens DM4 <br>
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| - | 5. insert dcmA into the pRSFDuet, and transformed this into DH5α cells for hypermutagenic PCR on the dcmA gene <br>
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| - | 6. fuse dcmA and sfGFP using PCR, followed by restriction and ligation to insert the dcmA-sfGFP transcriptional fusion into pRSFDuet. This was then transformed into DH5α cells and imaged using fluorescence microscopy <br>
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| - | 7. insert microcompartment-tagged dcmA into pRSFDuet<br>
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| - | 8. insert the microcompartment-tagged sfGFP into pME6010, and used fluorescence microscopy to image this<br>
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| - | <br><br>
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| - | <font style=“font-weight:600;”>Realisation Results:</font> <br><br>
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| - | For the containment of our bacteria, we have managed to: <br><br>
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| - | 1. synthesise novel agarose beads that have a polymeric coating which limits DCM diffusion into the beads. This allows optimum degradation by the bioremediation bacteria, while physically containing the bacteria for safety reasons <br>
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| - | 2. verify the functioning of the biopolymeric beads by measuring diffusion using indigo dye
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| - | 3. use computer-aided modelling to design a prototype of the DCMation system, and physically constructed this container <br>
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| - | 4. 3D print a cartridge to hold our biosensor bacteria, which can easily be replaced by the user<br>
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| - | 5. construct a prototype circuit that lights up when the photodiodes detect light emission from our biosensing bacteria that are contained in the cartridge. This lets the user have a simple yes/no response to whether the contents of the container are safe for disposal.<br>
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| - | <br>
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| - | <br>
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| | </div> | | </div> |
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"
The graph here is for non specific inputs and is for demonstration purposes only. It shows well how the model responds to changing the input values.
