From 2014.igem.org
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| - | <!--SYSUCHINA-->
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| - | <h1>DESIGN OVERVIEW</h1>
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| - | <p>In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
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| - | <p>
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| - | 1.The initiate state
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| - | </p>
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| - | <p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius</p>
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| - | <p>picture1</p>
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| - | <p>2.Infection</p>
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| - | <p>When the temperature rise to 30 degree Celcius, the M13 vector infects into the <em>E. coli</em>.</p>
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| - | <p>picture2</p>
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| - | <p>3.Mutagenesis</p>
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| - | <p>When the M13 vector undergo replication, a library of different Prey is generated.</p>
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| - | <p>picture3</p>
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| - | <p>4.Selection</p>
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| - | <p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p>
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| - | <p>picture4</p>
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| - | <p>5.Temperature regulated amplification</p>
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| - | <p>The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8</p>
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| - | <p>picture5</p>
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| - | <p>6. Rescue and Evolved</p>
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| - | <p>The p8 compensate M13 deficient, so that progeny phage can be generated</p>
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| - | <p>picture6</p>
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| - | <!--SYSUCHINA!-->
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Latest revision as of 23:02, 17 October 2014
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