Team:SYSU-China/file/Project/Design/Overview.html

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<h1>Overview</h1>
<h1>Overview</h1>
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<p>In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
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</p>
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<p>
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1.The initiate state
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</p>
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<p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius</p>
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<p>picture1</p>
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<p>2.Infection</p>
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<p>When the temperature rise to 30 degree Celcius, the M13 vector infects into the <em>E. coli</em>.</p>
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<p>picture2</p>
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<p>3.Mutagenesis</p>
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<p>When the M13 vector undergo replication, a library of different Prey is generated.</p>
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<p>picture3</p>
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<p>4.Selection</p>
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<p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p>
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<p>picture4</p>
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<p>5.Temperature regulated amplification</p>
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<p>The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8</p>
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<p>picture5</p>
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<p>6. Rescue and Evolved</p>
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<p>The p8 compensate M13 deficient, so that progeny phage can be generated</p>
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<p>picture6</p>
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Revision as of 22:58, 17 October 2014

Overview

In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.

1.The initiate state

M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius

picture1

2.Infection

When the temperature rise to 30 degree Celcius, the M13 vector infects into the E. coli.

picture2

3.Mutagenesis

When the M13 vector undergo replication, a library of different Prey is generated.

picture3

4.Selection

Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system

picture4

5.Temperature regulated amplification

The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8

picture5

6. Rescue and Evolved

The p8 compensate M13 deficient, so that progeny phage can be generated

picture6

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