Team:Penn/Protocol

From 2014.igem.org

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     <a href="https://2014.igem.org/Team:Penn/Protocol"><li>Protocols</li></a>
     <a href="https://2014.igem.org/Team:Penn/Protocol"><li>Protocols</li></a>
     <a href="https://2014.igem.org/Team:Penn/Supplement"><li>Supplementary Materials</li></a>
     <a href="https://2014.igem.org/Team:Penn/Supplement"><li>Supplementary Materials</li></a>
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    <a href="https://2014.igem.org/Team:Penn/Resources"><li>Resources</li></a>
 
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   <li>Team
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   <li>About
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   <ul>
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       <a href="https://2014.igem.org/Team:Penn/Team"><li>About Us</li></a>
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       <a href="https://2014.igem.org/Team:Penn/Team"><li>Team</li></a>
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                        <a href="https://2014.igem.org/Team:Penn/Achievements"><li>Achievements</li></a>
     <a href="https://2014.igem.org/Team:Penn/Sponsors"><li>Sponsors</li></a>
     <a href="https://2014.igem.org/Team:Penn/Sponsors"><li>Sponsors</li></a>
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    <a href="https://2014.igem.org/Team:Penn/Resources"><li>References</li></a>
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Revision as of 22:45, 17 October 2014

University of Pennsylvania iGEM

Protocols

Miniprep Cloning PCR Colony PCR Gel Extraction PCR Cleanup Restriction Enzyme Digest Ligation Transformation Inoculation E.Coli Specific Protocols

Gel Extraction

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Inoculation

Inoculation Long-Term Protocol

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques