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Team:Northwestern/CFPS
From 2014.igem.org
(Difference between revisions)
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<li>data from BL21 (E.Coli) lysates</li> | <li>data from BL21 (E.Coli) lysates</li> | ||
<li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | <li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | ||
| - | <li>the plasmid used was superfolder GFP from Jewett Lab</li> | + | <li>the plasmid used was a construct of superfolder GFP from the Jewett Lab</li> |
| - | <li><b>conclusion</b>our lysates are | + | <li><b>conclusion</b>our lysates are functional</li> |
</ul> | </ul> | ||
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<li>RBS B0034</li> | <li>RBS B0034</li> | ||
<li>Tried all of our lysates</li> | <li>Tried all of our lysates</li> | ||
| - | <li>green fluorescence indicates | + | <li>green fluorescence indicates mRNA levels of transcription</li> |
| - | <li>red fluorescence indicates | + | <li>red fluorescence indicates protein levels of translation</li> |
| - | <li><b>conclusion</b>: the data is most likely statistically | + | <li><b>conclusion</b>: the data is most likely statistically insignificant</li> |
</ul> | </ul> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-md-3"> | <div class="col-md-3"> | ||
| - | <h5>20141014 | + | <h5>20141014 Cell-Free Protein Synthesis Assay with All of our Lysates</h5> |
<ul> | <ul> | ||
| - | <li>We used RBS | + | <li>We used RBS No. 5 (B0034) with RFP-Spinach Aptamer</li> |
<li>Displayed here is the first read</li> | <li>Displayed here is the first read</li> | ||
<li>The kinetic portion of the plate reader did not record data in all of our wells</li> | <li>The kinetic portion of the plate reader did not record data in all of our wells</li> | ||
Revision as of 22:45, 17 October 2014
Cell-Free Protein Synthesis
20140723 Testing our lysates
- data from BL21 (E.Coli) lysates
- out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
- the plasmid used was a construct of superfolder GFP from the Jewett Lab
- conclusionour lysates are functional
20140915 All of our lysates
- RBS B0034
- Tried all of our lysates
- green fluorescence indicates mRNA levels of transcription
- red fluorescence indicates protein levels of translation
- conclusion: the data is most likely statistically insignificant
20141014 Cell-Free Protein Synthesis Assay with All of our Lysates
- We used RBS No. 5 (B0034) with RFP-Spinach Aptamer
- Displayed here is the first read
- The kinetic portion of the plate reader did not record data in all of our wells
- We will continue doing CFPS experiments
- conclusion: we need more time! Looks like fluorophores were incorrectly added
