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Team:SYSU-China/file/Project/Design/Overview
From 2014.igem.org
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<p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | <p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | ||
<p>picture3</p> | <p>picture3</p> | ||
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| + | <p>4.Selection</p> | ||
| + | <p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p> | ||
| + | <p>picture4</p> | ||
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| + | <p>5.Temperature regulated amplification</p> | ||
| + | <p>The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8</p> | ||
| + | <p>picture5</p> | ||
| + | |||
| + | <p>6. Rescue and Evolved</p> | ||
| + | <p>The p8 compensate M13 deficient, so that progeny phage can be generated</p> | ||
| + | <p>picture6</p> | ||
| + | <!--SYSUCHINA!--> | ||
Revision as of 22:41, 17 October 2014
DESIGN OVERVIEW
In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
1.The initiate state
M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius
picture1
2.Infection
When the temperature rise to 30 degree Celcius, the M13 vector infects into the E. coli.
picture2
3.Mutagenesis
When the M13 vector undergo replication, a library of different Prey is generated.
picture3
4.Selection
Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system
picture4
5.Temperature regulated amplification
The temperature rise to 37 degree Celcius, so that mRNA of gⅧ can be translated into p8
picture5
6. Rescue and Evolved
The p8 compensate M13 deficient, so that progeny phage can be generated
picture6
