Revision as of 22:40, 17 October 2014

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Genetic Parts and Measurement Kit

<groupparts>iGEM2014 Austin_Texas</groupparts>

BioBrick Parts

For iGEM 2014, the UT Austin team has biobricked two non-canonical amino acid (ncAA) tRNA synthetases with their cognate tRNAs. There are now three ncAA tRNA synthetases biobricked, including one from last year's UT Austin team.

[http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Austin_Texas Group Parts - 2014]
Group Parts - 2013 (related)

These parts have been tested and characterized as part of our ncAA kit project and our Photocage project.

ncAA Measurement Kit Plasmids

The 2014 UT Austin iGEM team felt quite strongly that we should make our ncAA reporter kit widely available as soon as possible. However, the pFYC and pFRY plasmids are meant to be a standardized measurement kit that is placed in a host cell alongside other plasmids (rather than a source of genetic parts that would be combined with other parts), so they are not standard BioBricks. We asked about an exception to place these plasmids in the Registry as a measurement device. In consultation with Vinoo Selvarajah (iGEM HQ), it was decided that, for now, we would include the relevant information here and share the kit plasmids (and cultures) with anyone who requests them ourselves, until the Registry is able to accommodate these types of devices.

We have included detailed information for our ncAA reporter kit here and also put the full sequences of these constructs on the Registry website.

The two key plasmids are:

pFRYC ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416003 BBa_K1416003]) - Reporter plasmid with linker containing tyrosine codon.
pFRY ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416004 BBa_K1416004]) - Reporter plasmid with linker containing amber codon.

In addition, you can ask us for one or more pStG plasmids like [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416002 BBa_K1416002] for different ncAAs, as a control, if you like.

Additional details for using the kit and experimental protocol can be found on our Expanded Genetic Code Measurement Kit page.

If you have questions about this kit or would like to receive it, please contact us at utigem [at] gmail [dot] com

Thank you for your interest in our biobrick parts and our measurement kit!

@@ Line 94: / Line 94: @@
 *pFRY ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416004 BBa_K1416004]) - Reporter plasmid with linker containing amber codon.
-In addition, you can ask us for one or more pStG plasmids [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416002 BBa_K1416002] for different ncAAs, as a control, if you like.
+In addition, you can ask us for one or more pStG plasmids like [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1416002 BBa_K1416002] for different ncAAs, as a control, if you like.
 Additional details for [https://2014.igem.org/Team:Austin_Texas/kit#Using_The_Kit using the kit] and [https://2014.igem.org/Team:Austin_Texas/kit#Experimental_Preparation experimental protocol] can be found on our [https://2014.igem.org/Team:Austin_Texas/kit Expanded Genetic Code Measurement Kit page].

Team:Austin Texas/parts

From 2014.igem.org

Revision as of 22:40, 17 October 2014

BioBrick Parts

ncAA Measurement Kit Plasmids