Team:StanfordBrownSpelman/BioBricks
From 2014.igem.org
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<h5 id="AHC"><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Amberless_Hell_Cell">Amberless Hell Cell</a></h5> | <h5 id="AHC"><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Amberless_Hell_Cell">Amberless Hell Cell</a></h5> | ||
<h6> | <h6> | ||
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499200"><u>Part BBa_K1499200</u></a>: uvsE</div> | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499200"><u>Part BBa_K1499200</u></a>: This part encodes uvsE, a putative UV damage endonuclease found in <i>D. radiodurans</i> that protects cells from radiation-induced DNA damage.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499201"><u>Part BBa_K1499201</u></a>: uracil glycosylase 1</div> | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499201"><u>Part BBa_K1499201</u></a>: This parts encodes uracil glycosylase 1, a protein involved in the base-excision repair pathway that protects cells from DNA damage by removing uracil residues from the DNA double helix.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499202"><u>Part BBa_K1499202</u></a>: uracil glycosylase 2</div> | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499202"><u>Part BBa_K1499202</u></a>: This parts encodes uracil glycosylase 2, a protein involved in the base-excision repair pathway that protects cells from DNA damage by removing uracil residues from the DNA double helix.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499203"><u>Part BBa_K1499203</u></a>: SdaB protein generator | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499203"><u>Part BBa_K1499203</u></a>: This part encodes the SdaB protein generator with 5 UAG stop codons incorporated at leucine residues. Serine deamine B is a protein involved in resistance to alkaline conditions.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499205"><u>Part BBa_K1499205</u></a>: MntH with | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499205"><u>Part BBa_K1499205</u></a>: This part encodes the manganese transporter MntH with a UAG stop codon in place of a leucine and a promoter for expression. MntH is involved with manganese homeostasis, a critical process for dealing with free radicals generated upon exposure to radiation that could potentially cause DNA damage.</div> |
<div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499250"><u>Part BBa_K1499250</u></a>: GFP (E0040) with two amber stop codons. This GFP contains two stop codons in place of leucine residues. In an amberless cell that also contains supP tRNA, GFP will be translated normally, otherwise, it will be truncated.</div> | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499250"><u>Part BBa_K1499250</u></a>: GFP (E0040) with two amber stop codons. This GFP contains two stop codons in place of leucine residues. In an amberless cell that also contains supP tRNA, GFP will be translated normally, otherwise, it will be truncated.</div> | ||
<div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499251"><u>Part BBa_K1499251</u></a>: tRNA supP. This is a UAG-leucine tRNA, so a leucine is added every time a UAG codon is encountered.</div> | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499251"><u>Part BBa_K1499251</u></a>: tRNA supP. This is a UAG-leucine tRNA, so a leucine is added every time a UAG codon is encountered.</div> |
Revision as of 22:32, 17 October 2014