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Team:SYSU-China/file/Project/Design/Overview
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Revision as of 22:31, 17 October 2014
DESIGN OVERVIEW
In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
1.The initiate state
M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius
picture1
2.Infection
When the temperature rise to 30 degree Celcius, the M13 vector infects into the E. coli.
picture2
3.Mutagenesis
When the M13 vector undergo replication, a library of different Prey is generated.
picture3
