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Team:Virginia/Safety
From 2014.igem.org
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| + | <li><b>Working with biosafety Level 2 organisms:</b> The Nylon Hydrolase biobrick was made over the course of the fall. The gene comes from BBa_K1357007- a flavobacterium plasmid. Hence, this falls under the biosafety level 2 category. 3 lab members were allowed to work in constructing the Nylon Hydrolase construct. These lab members had undergone the BSL 2 safety training before working in nylon hydrolase.</li> | ||
| + | <li><b>A UV illuminator was used</b> for viewing bands in agarose gels. UV rays are also a minor source of potential danger as high rates of radiation are carcinogenic. Lab members working with UV illuminators wore face shields, lab coats, and gloves at all times in order to prevent any exposure to UV light. Also, it was emphasized not to leave the UV board on longer than necessary. </li> | ||
| + | <li><b>RNA safety: </b>A designated area was specified for use with RNA. This space was sprayed with RNAse away before use. Any lab work involving RNA was conducted with a set of barriers tips and pipettes that were only used for RNA. </li> | ||
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| + | <h2>Waste Disposal</h2> | ||
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| + | <li>Solid waste: Any solid waste that might have come in contact with our bacterial cultures was stored in an autoclavable bag. This waste included: pipette tips, petri dishes, falcon tubes, Eppendorf tubes, pasteur pipette tips, 96-well plates with lids, and spin columns with collection tubes. This bag was autoclaved and sealed before being discarded as waste. </li> | ||
| + | <li> Liquid Waste: All liquid waste was collected in a vacuum Erlenmeyer flask. This included various bacteria cultures. The Erlenmeyer had bleach added to it. </li> | ||
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Revision as of 22:00, 17 October 2014
Safety
2014 Virginia IGEM took various measures to ensure safety in the lab. Proper training and safe handling means reduced risk of contamination for the user and the environment, and ensures reliability of experimental procedures.
The lab:
The lab was certified for use with biosafety level 2 organisms before any lab work was started. This was done by submitting a laboratory safety form to University of Virginia biosafety.
Lab work was started only after every lab member had received these safety certifications by the University of Virginia:
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- Working with biosafety Level 2 organisms: The Nylon Hydrolase biobrick was made over the course of the fall. The gene comes from BBa_K1357007- a flavobacterium plasmid. Hence, this falls under the biosafety level 2 category. 3 lab members were allowed to work in constructing the Nylon Hydrolase construct. These lab members had undergone the BSL 2 safety training before working in nylon hydrolase.
- A UV illuminator was used for viewing bands in agarose gels. UV rays are also a minor source of potential danger as high rates of radiation are carcinogenic. Lab members working with UV illuminators wore face shields, lab coats, and gloves at all times in order to prevent any exposure to UV light. Also, it was emphasized not to leave the UV board on longer than necessary.
- RNA safety: A designated area was specified for use with RNA. This space was sprayed with RNAse away before use. Any lab work involving RNA was conducted with a set of barriers tips and pipettes that were only used for RNA.
Waste Disposal
- Solid waste: Any solid waste that might have come in contact with our bacterial cultures was stored in an autoclavable bag. This waste included: pipette tips, petri dishes, falcon tubes, Eppendorf tubes, pasteur pipette tips, 96-well plates with lids, and spin columns with collection tubes. This bag was autoclaved and sealed before being discarded as waste.
- Liquid Waste: All liquid waste was collected in a vacuum Erlenmeyer flask. This included various bacteria cultures. The Erlenmeyer had bleach added to it.
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