Team:WashU StLouis/Project/collaboration

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<tr><td colspan="3"> <center> <h1> Collaboration </h1> </center> </td></tr>
 
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<p> We also collaborated with the University of Virginia's iGEM Team by helping them distribute their survey! For more information, contact them at virginia.igem@gmail.com </p>
 
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<tr>
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<td align="center" valign="top" width="45%">
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<p> We also collaborated with the University of Virginia's
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iGEM Team by helping them distribute their survey! For more
 +
information, contact them at virginia.igem@gmail.com </p>
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<style>
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#virginiabadge{
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background: url("https://static.igem.org/mediawiki/2014/6/6a/Virginia_Badge2.png");
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<div style="text-align: center;"><br>
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We also helped to characterize a few parts from the registry:<br>
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</div>
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<p style="text-align: justify;"><a
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href="http://parts.igem.org/Part:BBa_M30109:Experience#User_Reviews">BBa_M30109</a>
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<br>
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BBa_M30109 does NOT work AT ALL. Digesting the plasmid with the iGEM
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restriction enzyme yields the right band lengths but other than that
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the sequence is junk. We tried on numerous occasions to assemble a
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plasmid to activate our light sensor system. This set us back half of
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the summer because of the faulty part. We found a new part on the
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registry with the same genes that showed slightly more promise.&nbsp;</p>
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<p style="text-align: justify;"><a
 +
href="http://parts.igem.org/Part:BBa_K1017726:Experience#User_Reviews">BBa_K1017726</a>
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<br>
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BBa_K1017726 digests with the iGEM restriction enzymes well. We were
 +
able to assemble a plasmid by swapping out ori and resistance (we
 +
needed a different antibiotic) in order to co-transform with our
 +
system. There is differentiable results between our light and dark
 +
system, so the part likely has the genes as reported. However,
 +
digestion with known restriction enzyme sites based off the sequence
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provided gave gel smears rather than clear bands, so not 100% convinced
 +
that this part is as advertised. </p>
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</td>
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</tr>
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</tbody>
</table>
</table>
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<p> We also helped to characterize a few parts from the registry. <br>
 
-
<a href=http://parts.igem.org/Part:BBa_M30109:Experience#User_Reviews>BBa_M30109</a> <br>
 
-
BBa_M30109 does NOT work AT ALL. Digesting the plasmid with the iGEM restriction enzyme yields the right band lengths but other than that the sequence is junk. We tried on numerous occasions to assemble a plasmid to activate our light sensor system. This set us back half of the summer because of the faulty part. We found a new part on the registry with the same genes that showed slightly more promise. <br>
 
-
<a href=http://parts.igem.org/Part:BBa_K1017726:Experience#User_Reviews>BBa_K1017726</a> <br>
 
-
BBa_K1017726 digests with the iGEM restriction enzymes well. We were able to assemble a plasmid by swapping out ori and resistance (we needed a different antibiotic) in order to co-transform with our system. There is differentiable results between our light and dark system, so the part likely has the genes as reported. However, digestion with known restriction enzyme sites based off the sequence provided gave gel smears rather than clear bands, so not 100% convinced that this part is as advertised. </p>
 
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Latest revision as of 21:39, 17 October 2014



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