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Team:Groningen/Template/MODULE/Notebook/secretion/week11
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Revision as of 21:14, 17 October 2014
October 13 - October 16
goal: place the anti Pseudomonas Aeruginosa system in Lactococcus lactis NZ9800.
We digested the anti Pseudomonas aeruginosa system and pIL253 vector with EcoRI & PstI.
Then 20 ul of ligation mix was dialysed for 1 h with dH2O. the dialysed sample was transformed into L. lactis NZ9800 using electroporation.
