Team:UCLA/Notebook/Protocols

From 2014.igem.org

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<tr><td><br><b>Equipment for the collection of recombinant silk</b></br><a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment Protocol</a></td></tr>
 
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<tr><td><br> <b>List of Reagents</b></br>
 
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<a href="https://static.igem.org/mediawiki/2014/9/92/Reagent_Protocol.pdf">Reagent Protocol</a></td></tr>
 
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<tr><td><br> <b>PCR for MASP1 and MASP2 </b></br>
 
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<a href="https://static.igem.org/mediawiki/2014/archive/4/47/20140701232004%21IGEM_PCR_reaction_design.pdf">PCR Protocol</a></td></tr>
 
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<tr><td><br><b>Silk Fibroin Extraction</b></br><a href="https://static.igem.org/mediawiki/2014/a/a7/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%281%29.pdf">Silk Fibroin Extraction Protocol</a></td></tr>
 
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<tr><td><br> <b>Silk Films</b></br>
 
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<a href="https://static.igem.org/mediawiki/2014/a/a9/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%282%29.pdf">Silk Films Protocol</a></td></tr>
 
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<tr><td><br> <b>Electrospun Fibers</b></br>
 
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<a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun Fiber Protocol</a></td></tr>
 
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<tr><td><b>Silk Thread</b></br>
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<tr><td><b>Recombinant Silk Proteins using Head to Tail Method</b></br>
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<a href="https://static.igem.org/mediawiki/2014/6/6e/Silk_Thread_Protocols.pdf">Silk Thread Protocol</a>
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<a href="https://static.igem.org/mediawiki/2014/6/66/Recombinant_Silk_Protocol_Paper.pdf">Protocol Paper for Producing Recombinant Silk with Head to Tail Method</a><br/>
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<a href="https://static.igem.org/mediawiki/2014/a/ab/Equipment_Protocol.pdf">Equipment for Recombinant Silk Production</a><br/>
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<a href="https://static.igem.org/mediawiki/2014/9/92/Reagent_Protocol.pdf">Reagent List for Recombinant Silk Production</a><br/>
<br>Outline</br>
<br>Outline</br>
<ol>
<ol>

Revision as of 20:55, 17 October 2014

iGEM UCLA




Protocols
Recombinant Silk Proteins using Head to Tail Method
Protocol Paper for Producing Recombinant Silk with Head to Tail Method
Equipment for Recombinant Silk Production
Reagent List for Recombinant Silk Production

Outline
  1. Construction of the Synthetic Spider Silk Multimeric Gene
  2. Cloning of the spider silk multimer into the expression vector
  3. Gene Expression of pETsilk
  4. E.coli Cell Lysis
  5. Silk Protein Purification Using IMAC
  6. Dialysis
  7. Protein Analysis
  8. Amino Acid Analysis
  9. Overall: Timing of Procedure

Reference

Materials Fabrication
Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web.


Production of MASP1/2
Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.



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