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Team:Wageningen UR/project/low copy
From 2014.igem.org
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<section id="overview"> | <section id="overview"> | ||
<h1>Looking for low copy number plasmids</h1> | <h1>Looking for low copy number plasmids</h1> | ||
| - | <h2>Overview</h2> | + | <br> |
| + | <h2>Overview and Approach</h2> | ||
| - | <p> Our team faced several problems in determining the copy number of the different <a href="http://parts.igem.org/Help:Plasmid_backbones/Nomenclature" class="soft_link">iGEM standard backbones </a> available in the registry. We started by trying pSB1A3, and by our surprise more the obtained concentrations of the Minipreped plasmids was higher than pSB1K3. This was the main reason why we decided to compare the copy number of the different plasmids by growin 10 mL of culture until an Optical Density (OD) of 0.6, minipreped it and determine its optical density, as well as, run the samples in an agarose gel.</p> | + | <p> Our team faced several problems in determining the copy number of the different <a href="http://parts.igem.org/Help:Plasmid_backbones/Nomenclature" class="soft_link">iGEM standard backbones </a> available in the registry. We started by trying pSB1A3, and by our surprise more the obtained concentrations of the Minipreped plasmids was higher than pSB1K3. This was the main reason why we decided to compare the copy number of the different plasmids by growin 10 mL of culture until an Optical Density (OD) of 0.6, minipreped it and determine its optical density, as well as, run the samples in an agarose gel. All these was done in triplicates, except for the high copy number plasmid pSB1K3. </p> |
<h2>Results</h2> | <h2>Results</h2> | ||
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<img src="https://static.igem.org/mediawiki/2014/e/e4/Wageningen_UR_copynumbers.png" width="95%"> | <img src="https://static.igem.org/mediawiki/2014/e/e4/Wageningen_UR_copynumbers.png" width="95%"> | ||
<br></br> | <br></br> | ||
| - | <figcaption> Figure 1. a) b)</figcaption> | + | <figcaption> Figure 1. Copy number plasmid of the different plasmids of the iGEM registry. a) Representation of the minipreped concentrations obtained from the different copy number plasmids b) Gel electrophoresis of the different copy number plasmids. </figcaption> |
</figure> | </figure> | ||
<br></br> | <br></br> | ||
Revision as of 20:40, 17 October 2014
Looking for low copy number plasmids
Overview and Approach
Our team faced several problems in determining the copy number of the different iGEM standard backbones available in the registry. We started by trying pSB1A3, and by our surprise more the obtained concentrations of the Minipreped plasmids was higher than pSB1K3. This was the main reason why we decided to compare the copy number of the different plasmids by growin 10 mL of culture until an Optical Density (OD) of 0.6, minipreped it and determine its optical density, as well as, run the samples in an agarose gel. All these was done in triplicates, except for the high copy number plasmid pSB1K3.
Results
