Team:Evry/Notebook/Transposons/15.10.14

From 2014.igem.org

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14.10.2014 ligation gave only one clone.  
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== 14.10.2014 ligation==
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it gave only one clone.  
Clone culture:
Clone culture:
5 ml LB + 5 ul Kan (25 mg/ml) - 37 C°
5 ml LB + 5 ul Kan (25 mg/ml) - 37 C°
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== Transformation <i>Pseudovibrio denitrificans</i> & Dh5 alpha with mRFP in BBa_1413044==
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<br/>It parallel annotation of plasmid pNK2 with Blast is realized.
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electrocompetent cells:
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1 mm cuvette; 1800 V
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recuperation media: MB for <i>Pseudovibrio denitrificans</i> , LB for DH5alpha
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plating:
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MB agar for <i>Pseudovibrio denitrificans</i>
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LB agar for LB for DH5alpha
<img src="" width="50%"/>
<img src="" width="50%"/>
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& <i>Pseudovibrio denitrificans
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== Transformation <i>Pseudovibrio denitrificans</i> & Dh5 alpha with BBa_1413001 in BBa_1413044==
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electrocompetent cells:
 +
 +
1 mm cuvette; 1800 V
 +
recuperation media: MB for <i>Pseudovibrio denitrificans</i> , LB for DH5alpha
 +
 +
plating:
 +
 +
MB agar for <i>Pseudovibrio denitrificans</i>
 +
LB agar for LB for DH5alpha
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 +
<img src="" width="50%"/>
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<span class="cd-date">Aug 08</span>  
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<span class="cd-date">Oct 15</span>  
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Revision as of 20:32, 17 October 2014

Picture

== 14.10.2014 ligation== it gave only one clone. Clone culture: 5 ml LB + 5 ul Kan (25 mg/ml) - 37 C° == Transformation Pseudovibrio denitrificans & Dh5 alpha with mRFP in BBa_1413044== electrocompetent cells: 1 mm cuvette; 1800 V recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha plating: MB agar for Pseudovibrio denitrificans LB agar for LB for DH5alpha == Transformation Pseudovibrio denitrificans & Dh5 alpha with BBa_1413001 in BBa_1413044== electrocompetent cells: 1 mm cuvette; 1800 V recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha plating: MB agar for Pseudovibrio denitrificans LB agar for LB for DH5alpha

Oct 15

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