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Team:Groningen/Template/MODULE/Notebook/secretion/week9
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Revision as of 20:27, 17 October 2014
8 – 12 September
Goal: analysis of the possible made Biobricks.
Because of the very low amount of gblocks given, a decision was made to do a PCR directly on the product itself, therefor multiplying it exponentially.
All the O/N cultures were mini-prepped. The possible plasmids were cut with EcoRI and SpeI. After running on gel, the result showed us that 20 of the 40 clones contained all the Biobricks, though with a low yield of plasmids.
A new strategy of assembling has been prepared and primers for this strategy have been made.
All the Biobricks were send for sequencing.
