Team:INSA-Lyon/Results

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<div align=”center”><img src="https://static.igem.org/mediawiki/2014/0/0e/Adh%C3%A9rence.png" alt="Figure 1 : Engineered bacteria Percentage of adhesion"/></div>
<div align=”center”><img src="https://static.igem.org/mediawiki/2014/0/0e/Adh%C3%A9rence.png" alt="Figure 1 : Engineered bacteria Percentage of adhesion"/></div>
<b>Figure 1 : Engineered bacteria Percentage of adhesion</b><br/>
<b>Figure 1 : Engineered bacteria Percentage of adhesion</b><br/>
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<p align="justify"><i>csgA-</i>knockout <i>E. coli</i> strain was transformed with BBa_CsgA-WT (BBa_K1404006); BBa_CsgA-His1 (BBa_K1404007); BBa_CsgA-His2 (BBa_K1404008). The corresponding positive and negative controls are Wild-type <i>E.coli</i> curli producing strain transformed with with the empty vector and <i>csgA-</i>-knockout <i>E. coli</i> strain transformed with the empty vector respectively. <br/>
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<p align="justify"><i>csgA-</i>knockout <i>E. coli</i> strain was transformed with BBa_CsgA-WT (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>); BBa_CsgA-His1 (<a href="http://parts.igem.org/Part:BBa_K1404007">BBa_K1404007</a>); BBa_CsgA-His2 (<a href="http://parts.igem.org/Part:BBa_K1404008">BBa_K1404008</a>). The corresponding positive and negative controls are Wild-type <i>E.coli</i> curli producing strain transformed with with the empty vector and <i>csgA-</i>-knockout <i>E. coli</i> strain transformed with the empty vector respectively. <br/>
Strains with our parts, the positive and negative controls were cultivated in 24-wells microplate in M63 Mannitol during 24H at 30°C. The supernatant was removed and the OD600 measured, then the bacteria forming the biofilm were resuspended and the OD600 measured in order to estimate the number of cells (See protocol for more details). The percentage of adhesion was calculated as follow :  
Strains with our parts, the positive and negative controls were cultivated in 24-wells microplate in M63 Mannitol during 24H at 30°C. The supernatant was removed and the OD600 measured, then the bacteria forming the biofilm were resuspended and the OD600 measured in order to estimate the number of cells (See protocol for more details). The percentage of adhesion was calculated as follow :  
(OD600 of  the biofilm)/ (OD600 of  the supernatant + OD600 of the biofilm) <br/>
(OD600 of  the biofilm)/ (OD600 of  the supernatant + OD600 of the biofilm) <br/>

Revision as of 20:26, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization