<ol>

<li>Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.</li>

<li>add glycerol to culture to final concentration of 80% glycerol.</li>

<li>place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1.5ml to allow for expansion by freezing).</li>

</ol>

<li>cut two 3 inch PVC tubes .</li>

<li>sandwich them with nafion cation exchange membrane between them.</li>

<li>add caulking around both sides of membrane-tube junction.</li>

<li>let dry for 15 minutes.</li>

<li>repeat step 3 and 4.</li>

<li>cut off excess membrane around junction.</li>

<li>add one more layer to seal up junction.</li>

<li>let dry overnight.</li>

<li>cut 2.5in-3in x 12in of carbon cloth</li>

<li>roll around tip of finger.</li>

<li>weave titanium wire close to end edge of roll.</li>

<li>fold titanium wire up the length of the roll.</li>

<li>twist excess wire around itself.</li>

<li>cut wire at .5in-1in.</li>

<li>push through stopper.</li>

<li>seal hole from wire with caulking.</li>

<li>let dry overnight. (two of these make up the cathode and the anode)</li>

<li>pour potassium ferricyanide in one chamber of the cell.</li>

<li>carefully push stopper with carbon cloth to plug the chamber.</li>

<li>push hollow needle through stopper  to let air out of chamber.</li>

<li>pour overnight culture of MR-1 into other chamber.</li>

<li>place glass slide in chamber.</li>

<li>carefully push stopper with carbon cloth to plug the chamber.</li>

<li>push new sterile hollow needle in stopper into air bubble in chamber to let out any CO2 bacteria will produce.</li>

<li>open ExcelINX up on computer.</li>

<li>connect electrodes from DMM to cathode and anode (remember which channels are being used).</li>

<li>start ExcelINX.</li>