Team:UC-Santa Cruz-BioE/Notebook

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UC-Santa_Cruz-BioE/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:UC-Santa_Cruz-BioE"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:UC-Santa_Cruz-BioE/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=UC-Santa_Cruz-BioE"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:UC-Santa_Cruz-BioE/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:UC-Santa_Cruz-BioE/Attributions"style="color:#000000"> Attributions </a></td>
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<!..Code to edit the page. Don't Erase!..>
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<p><a href="https://2014.igem.org/wiki/index.php?title=Team:UC-Santa_Cruz-BioE/Notebook&action=edit"style="color:#FFFFFF; position:absolute; left:400px; top:62px; z-index:1; "> Click here  to edit this page!</a></p>
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<span style="color:#191970;font-family:Arial;font-size:16px;"><h1>Important Protocols</h1><!..A HEADING!..></span></div>
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<div id="paragraph1" style=" position: absolute; left:243px; top:474px; width 400px; height 200px;text-align=left;">
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<div class="edges";>
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<h3>Glycerol Stock Preparation</h3>
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</div>
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<div class="edges";>
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<ol>
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<li>Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.</li>
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<li>add glycerol to culture to final concentration of 80% glycerol.</li>
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<li>place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1.5ml to allow for expansion by freezing).</li>
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</ol>
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</div>
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</div>
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</td>
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<div id="paragraph2" style= "position: absolute; left:243px; top:674px; width:400px; height:200px; text-align=left;">
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<div class="edges";>
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<h3>Microbual Fuel Cell Assembly</h3>
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</div>
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<div class="edges";>
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<ol>
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<li>cut two 3 inch PVC tubes .</li>
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<li>sandwich them with nafion cation exchange membrane between them.</li>
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<li>add caulking around both sides of membrane-tube junction.</li>
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<li>let dry for 15 minutes.</li>
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<li>repeat step 3 and 4.</li>
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<li>cut off excess membrane around junction.</li>
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<li>add one more layer to seal up junction.</li>
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<li>let dry overnight.</li>
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<li>cut 2.5in-3in x 12in of carbon cloth</li>
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<li>roll around tip of finger.</li>
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<li>weave titanium wire close to end edge of roll.</li>
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<li>fold titanium wire up the length of the roll.</li>
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<li>twist excess wire around itself.</li>
 +
<li>cut wire at .5in-1in.</li>
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<li>push through stopper.</li>
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<li>seal hole from wire with caulking.</li>
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<li>let dry overnight. (two of these make up the cathode and the anode)</li>
 +
<li>pour potassium ferricyanide in one chamber of the cell.</li>
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<li>carefully push stopper with carbon cloth to plug the chamber.</li>
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<li>push hollow needle through stopper  to let air out of chamber.</li>
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<li>pour overnight culture of MR-1 into other chamber.</li>
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<li>place glass slide in chamber.</li>
 +
<li>carefully push stopper with carbon cloth to plug the chamber.</li>
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<li>push new sterile hollow needle in stopper into air bubble in chamber to let out any CO2 bacteria will produce.</li>
 +
<li>open ExcelINX up on computer.</li>
 +
<li>connect electrodes from DMM to cathode and anode (remember which channels are being used).</li>
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<li>start ExcelINX.</li>
 +
</ol>
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</div>
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</div>
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<h3>Plasmid Prep</h3>
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</div>
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</div>
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<!--requirements section -->
 
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
 
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
 
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Revision as of 20:04, 17 October 2014


Notebook

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notebooknotebook

Important Protocols

Glycerol Stock Preparation

  1. Inoculated TSB with MR-1 (either from plate or glycerol stock) and grow overnight.
  2. add glycerol to culture to final concentration of 80% glycerol.
  3. place 1ml in 1.5ml freezer safe eppendorf tubes (do not fill eppendorfs with more than 1.5ml to allow for expansion by freezing).

Microbual Fuel Cell Assembly

  1. cut two 3 inch PVC tubes .
  2. sandwich them with nafion cation exchange membrane between them.
  3. add caulking around both sides of membrane-tube junction.
  4. let dry for 15 minutes.
  5. repeat step 3 and 4.
  6. cut off excess membrane around junction.
  7. add one more layer to seal up junction.
  8. let dry overnight.
  9. cut 2.5in-3in x 12in of carbon cloth
  10. roll around tip of finger.
  11. weave titanium wire close to end edge of roll.
  12. fold titanium wire up the length of the roll.
  13. twist excess wire around itself.
  14. cut wire at .5in-1in.
  15. push through stopper.
  16. seal hole from wire with caulking.
  17. let dry overnight. (two of these make up the cathode and the anode)
  18. pour potassium ferricyanide in one chamber of the cell.
  19. carefully push stopper with carbon cloth to plug the chamber.
  20. push hollow needle through stopper to let air out of chamber.
  21. pour overnight culture of MR-1 into other chamber.
  22. place glass slide in chamber.
  23. carefully push stopper with carbon cloth to plug the chamber.
  24. push new sterile hollow needle in stopper into air bubble in chamber to let out any CO2 bacteria will produce.
  25. open ExcelINX up on computer.
  26. connect electrodes from DMM to cathode and anode (remember which channels are being used).
  27. start ExcelINX.

Plasmid Prep

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