"
Team:ETH Zurich/expresults/qs/tab-prhl
From 2014.igem.org
| Line 3: | Line 3: | ||
|colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. | |colspan="4" style='font-size:10pt'|In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 10<sup>3</sup> nM. | ||
| - | + | In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed. | |
| - | + | ||
Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green. | Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 10<sup>3</sup> nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green. | ||
|- | |- | ||
Revision as of 20:04, 17 October 2014
| In this set of experiment the promoter pRhl was tested for possible crosstalk. In the top row left graph we see the the induction of pRhl by C4-HSL bound to regulator RhlR. The switching behaviour was observed at a concentration of the inducer molecule of 103 nM.
In the case of 3OC12-HSL binding the RhlR regulator and subsequently the promoter pRhl negligible crosstalk has been observed. Severe crosstalk was detected in the case of 3OC6-HSL binding the RhlR regulator followed by induction of pRhl. The transition occurred at a concentration of the inducer molecule of 103 nM but compared to the reference curve a lower (1000 AU) value of fluorescence was observed. The second case of crosstalk with the pRhl was detected with 3OC12-HSL binding to the corresponding LasR regulator followed by inducing the promoter pRhl. There the switching occurred at a concentration 1nM of 3OC12-HSL reaching a fluorescence of 750 AU. This is approximately 0.5 fold the value of the fluorescence shown by the reference curve in green. | |||
| 
| 
| 
|
| 
| 
| 
|
| 
| 
| 
|
| 
| 
| 
|
