Team:TCU Taiwan/Safety

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     <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">Safety</font></h6></td>
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     <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">Achievements</font></h6></td>
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       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
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      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Achievements" style="color:#DBAC52"><font size="3">Achievements</font></a></li>
       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
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   <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety" style="color:#DBAC52"><font size="5">Safety</font></a></li>
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   <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety"><font size="5">Safety</font></a></li>
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    <li>Form a team.</li>
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    <li>Complete judging form.</li>
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    <li>Constructed attractive <a href="https://2014.igem.org/Team:TCU_Taiwan">Wiki</a> page. </li>
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    <li>Present a poster and a talk were readied for Giant Jamboree.</li>
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    <li>Several Biobricks were built, improved and documented by our team.</li>
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-<a href="https://2014.igem.org/Team:TCU_Taiwan/Parts">Construct new BioBricks</a><br>a. 19 gRNAs
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<br>b. 1 phagemid pBluescript
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<br>c. 1 pRha induced CRISPR
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        <font size="3" face="Verdana" color="#D92332"><li>ENJOY THE iGEM! </li></font>
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        <font size="3" face="Verdana" color="#333"><li>All team member has to take lab safety  training.</li></font>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">1.Safety Training</font><br>
 
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  <p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>a)</strong></font></span><strong><font size="3" face="Verdana" color="#333">H</font></strong><font size="3" face="Verdana" color="#333"><strong>ave your team members received any safety training yet?</strong></font></p>
 
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<div><li><label for="yes_had_training"><font size="3" face="Verdana" color="#333">Yes, we have already received safety training.</font></label></li></div>
 
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>b)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Please briefly describe the topics that you learned about in your safety training.</font></strong><font size="3" face="Verdana" color="#333"></font></p>
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<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333">1.Universal lab safety rules: wearing lab coat and glasses, washing hands, keeping work area uncluttered, etc.<br>
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  2.Know the location of fire extinguisher, eyewash station, chemical spill kits and else<br>
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  3.First aid lesson<br>
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  4.Help yourself: memorizing emergency safety telephone number</font></div>
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<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">2.Local Rules and Regulations</font></legend>
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>a)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Who is responsible for biological safety at your institution?</font></strong><font size="3" face="Verdana" color="#333"></font></p>
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<div class="wrapper" style="background-color: white;"><ul style="margin-left: 1em;"><font size="3" face="Verdana" color="#333"><li><a href="http://www.eps.tcu.edu.tw/" target="_blank">Environmental Health and Safety Center</a> in Tzu Chi University.</li></font></ul></div><br>
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<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">3.About Our Lab</font></legend>
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>a)</strong></font></span><strong><font size="3" face="Verdana" color="#333">What is the Safety Level of your lab? (Use the <a href="https://2014.igem.org/Safety_Hub#rgbsl" target="_blank">WHO numbering system</a>, in which Level 4 is used for the most dangerous organisms.)</font></strong><font size="3" face="Verdana" color="#333"></font></p>
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<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li><a href="https://2014.igem.org/Safety_Hub#safety_levels" target="_blank">Level 2</a> (moderate risk, &#8780; WHO BSL 2)</li></ul></font></div>
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>b)</strong></font></span><strong><font size="3" face="Verdana" color="#333">According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab? </font></strong></p>
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<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>Laminar flow hood/biosafety cabinet with open front
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     <td align="center"><font color="#333" size="2" face="Verdana">Cute BoBo looks back with a smile.</font></td>
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>c)</strong></font></span><strong><font size="3" face="Verdana" color="#333">What personal protective equipment do you use in your lab? Please check all that apply.</font></strong></p>
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     <font size="3" face="Verdana" color="#333"><li>Experimentally validate Biobricks of our team design and  construct were as expected.</li>
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      <label for="yes_had_training3"><font size="3" face="Verdana" color="#333"> Lab coats</font></label>
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       -<a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign">Construct  new system designs</a><br>
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    </li>
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        a. CRISPR  Construction (Figure.1)<br>
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        b. Recombinant  Phagemid Construction (Figure.2)
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       <label for="yes_had_training3"><font size="3" face="Verdana" color="#333">Gloves</font></label>
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     </ul>
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     <li>We have documented and  characterized of our <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts">parts</a> in the &rdquo;Main Page&rdquo; section and registry entry.</li>
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      <label for="yes_had_training3"><font size="3" face="Verdana" color="#333">Safety glasses / goggles</font></label>
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      <p>-Tests the BioBrick<br>
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        a. <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1473005">BBa_K1473005</a><br>
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        b. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473002</a><br>
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      <label for="yes_had_training3"><font size="3" face="Verdana" color="#333">Surgical masks</font></label>
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        c. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473003</a><br>
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        d. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473004</a></p>
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    <li>Submit our new parts to iGEM Parts registry
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<li>Because we use phages in our projct, it could cause some safety problems if phages remain in environment. So we decided to use helper phage and phagemid for phage display, this will solve these problems because those phages cannot have next generation.
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<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>d)</strong></font></span><strong><font size="3" face="Verdana" color="#333">How do you dispose of biological waste?</font></strong></p>
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<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>Liquid waste will be collected first and then sent to our school's liquid waste storing house.</li><li>Other waste will be collected into Biohazard waste disposal.</li></ul></font></div>
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<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">4.The Organisms and Parts</font></legend>
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<p><font size="3" face="Verdana" color="#333">Species name (including strain) Risk Group Risk Group Source Disease risk to humans? Part number/name Natural function of part How did you acquire it? How will you use it?</font></p>
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<p>&nbsp;</p><font size="3" face="Verdana" color="#A66B38">TCU Taiwan Safety2014 Spreadsheet</font>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38">Gold</font><br>
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    <li>We improve the function of BBa_K1218011  from 2013 <a href="http://parts.igem.org/Part:BBa_K1218011">Stanford-Brown</a> .</li>
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  </ul>
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    <li>Cooperation <a href="https://2014.igem.org/Team:NCTU_Formosa/human_practice ">with NCTU-Formosa</a> Team on  modeling. We provide the data to NCTU-Formosa Team then they make the model for  us.</li>
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    <li>In  our project, we still need antibiotics to reduce the amount of bacteria. So we  come up with an idea : use 16S rRNA as CIRSPR's target to avoid co-effect of  antibiotic use. We have put it in our future work. </li>
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     <td><font color="#0092C7">Species name</font></td>
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    <td><font color="#0092C7">Risk Group Source</font></td>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" style="height: 40px; float:inherit; margin-right: 5px;"></a>Human Practice</font><br>
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    <td><font color="#0092C7">Disease risk to humans?</font></td>
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    <td><font color="#0092C7">How did you acquire it?</font></td>
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  <font size="3" face="Verdana" color="#333"><ul>
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    <li>
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      Held an iGEM speech at National Hualien  Girl&rsquo;s Senior High School,  the content includes  introduction of  synthetic biology, our project. We have also designed a game about basic of our gRNA.<br>
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        <li>Join the 2014 iGEM Conference in National Chiao Tung University in summer vacation, we have social contacts with <a href="https://2014.igem.org/Team:NCTU_Formosa/human_practice#Team_Exchange">several iGEM teams from China and  Taiwan</a><br>
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        <li>Share Project and Promote Synthetic Biology in Tzu Chi University <br>
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        <li>Question Survey about synthetic biology.<br>
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        <li>Promotion of iGEM and Synthetic Biology to populace.<br>
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        <li>Face Page : <a href="https://www.facebook.com/TCUiGEM">Tzu Chi University iGEM</a>  <br>
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        <li>Skype with <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics">NCTU-Formosa</a> and <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics">NYMU-Taipei</a>
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     </li>
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     <td><em>E.coli</em> top 10</td>
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    <td>1</td>
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    <td>WHO</td>
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    <td>do not cause disease in healthy adult humans.
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    <td>Acquired from our lab
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    <td>Competent cell for our plasmid
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+
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><em>E.coli</em> JM101</td>
+
     <td>
-
    <td>1</td>
+
    <fieldset>
-
    <td>WHO</td>
+
  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling"><img src="https://static.igem.org/mediawiki/2014/3/3f/TCU-Modeling-logo.png" style="height: 40px; float:inherit; margin-right: 5px;"></a>Computer Modeling</font><br>
-
     <td>do not cause disease in healthy adult humans.
+
  <font size="3" face="Verdana" color="#333"></font></legend>
-
</td>
+
<div class="wrapper" style="background-color: white;">
-
     <td>Acquired from another lab in our school
+
  <font size="3" face="Verdana" color="#333"><ul>
-
</td>
+
     <li>
-
     <td>Model organism for infection by M13KO7
+
      find the best release amount of phage after helper  phage infecting.<br>
-
</td>
+
        <li>To know under which condition can get the best infection  rate of our phagemid-carrying phage.
 +
     </li>
 +
  </ul></font>
 +
</div>
 +
     </fieldset>
 +
    </td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><em>E.coli</em> DH5α</td>
+
     <td height="30px">&nbsp;</td>
-
    <td>1</td>
+
-
    <td>WHO</td>
+
-
    <td>do not cause disease in healthy adult humans.
+
-
</td>
+
-
    <td>Acquired from our lab
+
-
</td>
+
-
    <td>Competent cell for our plasmid
+
-
</td>
+
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><em>E.coli</em> NEB10 β</td>
+
     <td>
-
    <td>1</td>
+
    <fieldset>
-
    <td>WHO</td>
+
  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><a href="https://2014.igem.org/Team:TCU_Taiwan/Safety"><img src="https://static.igem.org/mediawiki/2014/2/21/TCU-Safety-logo.png" alt="" style="height: 40px; float:inherit; margin-right: 5px;"></a>Safety</font><br>
-
     <td>do not cause disease in healthy adult humans.
+
  <font size="3" face="Verdana" color="#333"></font></legend>
-
</td>
+
<div class="wrapper" style="background-color: white;">
-
    <td>Acquired from igem HQ
+
  <ul>
-
</td>
+
     <font size="3" face="Verdana" color="#333"><li>
-
     <td>Competent cell for our biobrick
+
      In our experiment, we choose  M13 phage as model. This filamentous phage will make its genome a double strand  plasmid in host bacteria&rsquo;s cytosol. Therefore, M13 phage is not either a lytic  or lysogenic phage, its release will not cause host cell&rsquo;s death.
-
</td>
+
    </li></font>
 +
  </ul></div>
 +
     </fieldset>
 +
    </td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>M13KO7 hlper phage</td>
+
     <td height="30px">&nbsp;</td>
-
    <td>1</td>
+
-
    <td>WHO</td>
+
-
    <td>do not cause disease in healthy adult humans.
+
-
</td>
+
-
    <td>Bought from NEB
+
-
</td>
+
-
    <td>Vector for our system
+
-
</td>
+
   </tr>
   </tr>
-
</table>
+
  <tr>
-
      
+
     <td height="30px">&nbsp;</td>
-
    </td>
+
   </tr>
   </tr>
-
</table>
 
-
<p align="right"><a href="https://static.igem.org/mediawiki/2014/1/18/TCU_Taiwan_Safety2014_Spreadsheet.xls" title="Download TCU Taiwan Safety2014 Spreadsheet"><img src="https://static.igem.org/mediawiki/2014/3/36/TCU_Excel_15.png" width="35px"></a></p>
 
-
<br>
 
-
<div class="inlinebox">
 
-
    <div id="spreadsheethelp" style="display: none;">
 
-
        <p><strong>Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".</strong></p>
 
-
        <ol style="list-style-type:upper-alpha">
 
-
            <li><strong>Species name (including strain):</strong> For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.</li>
 
-
            <li><strong>Risk Group:</strong> Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)</li>
 
-
            <li><strong>Risk Group Source:</strong> Cite the source from which you obtained the Risk Group information. See <a href="https://2014.igem.org/Safety/Risk_Group_Guide#rghowto">Risk Group Guide</a> for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.</li>
 
-
            <li><strong>Disease risk to humans?:</strong> Does this organism cause any disease in humans? If yes, what disease does it cause?</li>
 
-
            <li><strong>Part number/name:</strong> For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.</li>
 
-
            <li><strong>Natural function of part:</strong> For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.</li>
 
-
            <li><strong>How did you acquire it?:</strong> Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)</li>
 
-
            <li><strong>How will you use it?:</strong> Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")</li>
 
-
            <li><strong>Notes:</strong> Use this column to give any additional information that is necessary.</li></ol></div></div>
 
-
</fieldset>
 
-
<fieldset>
 
-
<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">5.Risks of Our Project Now</font></legend>
 
-
<br>
+
</table>
-
<p class="qbody">Please describe risks of working with the biological materials that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done to minimize them, instead of simply saying that there are no risks at all.</p>
+
   
-
<br>
+
</td>
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>a)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Risks to the safety and health of team members, or other people working in the lab:</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>Some reagents we use are poison for human. Such as EtBr, chloroform, etc.</li></ul></font></div>
+
-
<br>
+
-
<br>
+
-
 
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>b)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Risks to the safety and health of the general public (if any biological materials escaped from your lab):</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>As our mechanism includes phage therapy, there might be some dangerous for injecting phage into human body.</li></ul></font><br>
+
-
</div>
+
-
<br>
+
-
<br>
+
-
 
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>c)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Risks to the environment (from waste disposal, or from materials escaping from your lab):</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>CRISPR system may destroy bacteria's genome, thus break the balance of bacteria's population.</li><br><li>
+
-
  We try to engineer our gRNA to make it target only antibiotics resistant gene.</li></ul></font></div>
+
-
<br>
+
-
<br>
+
-
 
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>d)</strong></font></span><strong><font size="3" face="Verdana" color="#333">Risks to security through malicious mis-use by individuals, groups, or countries:</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>May used to cause unnecessary bacteria death in environment, result in  break of bacteria balance.</li></ul></font></div>
+
-
<br>
+
-
<br>
+
-
 
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>e)</strong></font></span><strong><font size="3" face="Verdana" color="#333">What measures are you taking to reduce these risks?</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333">1.We use helper phage and phagemid for phage display.<br>
+
-
  2.We try to engineer our gRNA to make it target only antibiotics resistant gene.</font></div>
+
-
<br>
+
-
</fieldset>
+
-
 
+
-
<fieldset>
+
-
<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">6.Risks of Our Project in the Future</font></legend>
+
-
<br>
+
-
<p>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</p>
+
-
 
+
-
<br>
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>a)</strong></font></span><font size="3" face="Verdana" color="#333"><strong>What <i>new</i> risks might arise from your project's growth?</strong></font></p>
+
-
<p class="qbody"><strong><font size="3" face="Verdana" color="#333">Also, what risks might arise if the <i>knowledge</i> you generate or the <i>methods</i> you develop became widely available?</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;"><ul style="margin-left: 1em;"><li><font color="#333" size="3" face="Verdana">Injecting phage into human body may cause some unknown danger.</font></li><li><font color="#333" size="3" face="Verdana">
+
-
  May speed up <em>E.coli</em> to develop &quot;resistance&quot; to phage.</font></li></ul></div>
+
-
<br>
+
-
<br>
+
-
 
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"><strong>b)</strong></font></span><font size="3" face="Verdana" color="#333"><strong>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?Such features are not required for an iGEM project, but many teams choose to explore them.</strong></font></p>
+
-
<div class="wrapper" style="background-color: white;"><font size="3" face="Verdana" color="#333"><ul style="margin-left: 1em;"><li>We thought to change phage's coat protein so we can access our phage's infection ability. But this is beyond our current project.</li></ul></font></div>
+
-
<br>
+
-
</fieldset>
+
-
 
+
-
<fieldset>
+
-
<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">7.Health Worker</font></legend>
+
-
<br>
+
-
<br>
+
-
<p class="qbody"><font size="3" face="Verdana" color="#333"><img src="https://static.igem.org/mediawiki/2014/f/f5/TCU_Health_Work.png" style=" float: left; margin-right: 5px;" width="135"><img src="https://static.igem.org/mediawiki/2014/4/48/TCU_SAFETY_10489164_882403321776060_1876998885_n.jpg" width="100" style=" float: right; margin-right: 5px;margin-left: 5px;">Emergency Medical Technician (EMT) are terms used in some countries to denote a <a href="http://en.wikipedia.org/wiki/Health_professional" target="_blank">health care provider</a> of <a href="http://en.wikipedia.org/wiki/Emergency_medical_services" target="_blank">emergency medical services</a>.EMTs are clinicians, trained to respond quickly to emergency situations regarding medical issues, traumatic injuries and accident scenes.</font></p>
+
-
<p class="qbody"><font size="3" face="Verdana" color="#333">House,one of our team members,is a Emergency Medical Technician.He got the EMT-1(EMT-B) Licence and he volunteered for Hualien County Fire Department.He can handle every medical emergencies for team members in daily life.</font></p>
+
-
 
+
-
<div class="wrapper" style="background-color: white;"></div>
+
-
 
+
-
<br>
+
-
</fieldset>
+
-
 
+
-
<fieldset>
+
-
<legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#90B849">8.Project safety</font></legend>
+
-
<br>
+
-
<p class="qbody"><span class="qnum"><font size="3" face="Verdana" color="#90B849"></font></span><strong><font size="3" face="Verdana" color="#333">As an environment project, the mean idea of our project’s safety is “not kill them but remove their resistance”, so here are some points.</font></strong><font size="3" face="Verdana" color="#333"></font></p>
+
-
<div class="wrapper" style="background-color: white;">
+
-
  <font size="3" face="Verdana" color="#333"><ol>
+
-
    <li>We choose phage as our vector  for the CRISPR system because of the high specificity. Each kind of phage will  only infect one kind of bacteria efficiently. </li><br>
+
-
    <li>To avoid the co-effect of phage  infection, we choose the &ldquo;phagemid-helper phage&rdquo; system for phage display. This  system creates progeny who cannot produce next generation along. Thus, our  project will not cause additional phage remaining in the environment.</li><br>
+
-
    <li>In our experiment, we choose  M13 phage as model. This filamentous phage will make its genome a double strand  plasmid in host bacteria&rsquo;s cytosol. Therefore, M13 phage is not either a lytic  or lysogenic phage, its release will not cause host cell&rsquo;s death.</li><br>
+
-
    <li>We design our CRISPR&rsquo;s target  as antibiotics resistance gene. So it will not influence bacteria&rsquo;s growth as  long as we don&rsquo;t add antibiotics. We did this because we want the situation is  always under control. (We DO NOT want to simply kill those bacteria, we just  want to remove their resistance and make them under our control.)</li>
+
-
  </ol></font>
+
-
</div><br>
+
-
</fieldset>
+
-
 
+
-
</div>
+
-
</tr>
+
-
<tr>
+
-
  <td colspan="3" height="30px" align="center"> 
+
-
</tr>
+
-
<tr>
+
-
  <td colspan="3" height="1px" align="center" width="70%"><strong><font face="Trebuchet MS" size="6" color="#A66B38"><p>TCU_Taiwan iGEM Laboratory Practices</p></font></strong>
+
   </tr>
   </tr>
 +
</table>
-
   
 
-
<tr>
 
-
  <td colspan="3" height="1px">
 
-
 
 
-
  <table width="70%" border="0" cellspacing="0" cellpadding="2" align="center">
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC">&nbsp;</td>
 
-
      <td bgcolor="#FCEABC">&nbsp;</td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td width="30px" align="right"><font size="3" face="Verdana" color="#333">1.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Don&rsquo;t eat, drink, prepare or store food, smoke, handle contact lenses or apply cosmetics in any laboratory.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">2.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Know where the nearest eyewash, safety shower, and fire extinguisher are located. Know how to use them.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">3.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Insist upon good housekeeping in your laboratory.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">4.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Check for insects and rodents. Keep all areas clean.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">5.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Secure all gas cylinders.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333"><font size="3" face="Verdana" color="#333">6.</font></font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Wear laboratory coats and other appropriate protective clothing while performing laboratory activities. Feet and legs should be covered; sandals and open-toed shoes should not be worn in laboratories. Wear appropriate gloves while handling infectious or toxic materials and animals. Do not wear lab coats. Gloves or other personal protective equipment outside the laboratory.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">7.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Use a biological safety cabinet for handling infectious materials or materials requiring protection from contamination and a fume hood for toxic materials; mixed hazards need to be evaluated case by case.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">8.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Fume hoods should be used for laboratory activities that could result in chemical explosions or fires, for experiments involving toxic, hazardous or carcinogenic compounds, and use of strong acids and bases. Biological safety cabinets should not be used for this kind of work. Fume hoods are workstations, not storage cabinets. Vented storage areas may be located under the fume hood work area. However, these are not for storage of flammables.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">9.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Respect chemicals and radionuclides. Know their hazards and follow appropriate safety precautions. Chemical and radioactive waste must not be poured down the drain.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">10.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">All equipment must be documented to be free of chemical, biological, and radiological contamination before repair work is done or before moving equipment for storage or elsewhere.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">11.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Never mouth pipette anything. Use mechanical pipetting devices only!</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">12.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Close laboratory doors while experiments are in progress. Restrict access to laboratory.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">13.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Put liquid traps and in-line vacuum filters on all vacuum lines.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">14.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Minimize or contain all aerosol-producing activities, large-volume work, concentrated solutions or cultures. These activities include centrifugation (use safety cups), vortex missing (stopper tube), blending (use metal safety blender), sonication, grinding, opening containers of infectious material, inoculating culture flasks, inoculating animals, harvesting infectious materials from cultures or animals, and weighing or reconstructing toxic powders, etc.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">15.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Place biological safety cabinets in low-traffic areas and minimize activities that disrupt air flow in or around cabinet.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">16.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Decontaminate all work surfaces daily, and decontaminate all spills immediately.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right"><font size="3" face="Verdana" color="#333">17.</font></td>
 
-
      <td><font size="3" face="Verdana" color="#333">Decontaminate (by autoclaving or chemical disinffection) all biologically contaminated materials – glassware, animal cages, laboratory equipment, etc. – before washing, reuse or disposal. Discard materials via proper waste stream.</font></td>
 
-
    </tr>
 
-
    <tr>
 
-
      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">18.</font></td>
 
-
      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Be careful with needles and syringes. Use only when alternative methods are not feasible.</font></td>
 
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      <td align="right"><font size="3" face="Verdana" color="#333">19.</font></td>
 
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      <td><font size="3" face="Verdana" color="#333">Syringes, needles, Pasteur pipettes, etc, should be placed in rigid, leak-proof containers (Sharps Safe) and discarded following the waste rules.</font></td>
 
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      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">20.</font></td>
 
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      <td bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">Broken glassware and disposable pipettes (after decontamination) should be placed in a &ldquo;Disposable Labware and Broken Glass Box&rdquo; and discarded following the waste rules.</font></td>
 
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      <td align="right"><font size="3" face="Verdana" color="#333">21.</font></td>
 
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      <td><font size="3" face="Verdana" color="#333">Place contaminated biological materials in covered, leak-proof containers before removing them from the laboratory.</font></td>
 
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      <td align="right" bgcolor="#FCEABC"><font size="3" face="Verdana" color="#333">22.</font></td>
 
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      <td align="right"><a href="https://static.igem.org/mediawiki/2014/4/40/TCU_Taiwan_iGEM_Laboratory_Practices_PDF.pdf" title="TCU Taiwan iGEM Laboratory Practices" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/5e/TCU_PDF.png" width="35"></a></td>
 
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  <td colspan="3" height="10px"  bgcolor="#FFF2B5" align="center"> <font face="Trebuchet MS" size="6" color="#A66B38">Trogene Horse's Hometown</font> 
 
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  <td colspan="3" align="center">  <iframe width="720" height="482" frameborder="0" src="http://files.slidemypics.com/app/js/iframe.html?bg_color=ffffff&amp;hash=1492090f52dc147797a13b1ca1459908&amp;r=0.6832032252568752"></iframe>
 
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     <td><img src="https://static.igem.org/mediawiki/2014/c/c1/TCU-Line.png" width="100%"></td>
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Revision as of 19:53, 17 October 2014



 
Achievements
  
 
Bronze
  • Form a team.
  • Complete judging form.
  • Constructed attractive Wiki page.
  • Present a poster and a talk were readied for Giant Jamboree.
  • Several Biobricks were built, improved and documented by our team.

 
 
Silver
  • Experimentally validate Biobricks of our team design and construct were as expected.
  • We have documented and characterized of our parts in the ”Main Page” section and registry entry.
  • Submit our new parts to iGEM Parts registry 
  • Because we use phages in our projct, it could cause some safety problems if phages remain in environment. So we decided to use helper phage and phagemid for phage display, this will solve these problems because those phages cannot have next generation.

 
Gold
  • Cooperation with NCTU-Formosa Team on modeling. We provide the data to NCTU-Formosa Team then they make the model for us.
  • In our project, we still need antibiotics to reduce the amount of bacteria. So we come up with an idea : use 16S rRNA as CIRSPR's target to avoid co-effect of antibiotic use. We have put it in our future work.
 
Human Practice
  • Held an iGEM speech at National Hualien Girl’s Senior High School, the content includes introduction of synthetic biology, our project. We have also designed a game about basic of our gRNA.
  • Join the 2014 iGEM Conference in National Chiao Tung University in summer vacation, we have social contacts with several iGEM teams from China and Taiwan
  • Share Project and Promote Synthetic Biology in Tzu Chi University
  • Question Survey about synthetic biology.
  • Promotion of iGEM and Synthetic Biology to populace.
  • Face Page : Tzu Chi University iGEM
  • Skype with NCTU-Formosa and NYMU-Taipei
 
Computer Modeling
  • find the best release amount of phage after helper phage infecting.
  • To know under which condition can get the best infection rate of our phagemid-carrying phage.
 
Safety
  • In our experiment, we choose M13 phage as model. This filamentous phage will make its genome a double strand plasmid in host bacteria’s cytosol. Therefore, M13 phage is not either a lytic or lysogenic phage, its release will not cause host cell’s death.
 
 
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Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

Lost the way? Use it to help you if you're lost.

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Retrieved from "http://2014.igem.org/Team:TCU_Taiwan/Safety"