Team:Austin Texas/parts

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Revision as of 19:45, 17 October 2014

UT Austin HOME	PROJECTS			HUMAN PRACTICES	OFFICIAL REQUIREMENTS
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Genetic Parts and Measurement Kit

<groupparts>iGEM2014 Austin_Texas</groupparts>

BioBrick Parts

For iGEM 2014, the UT Austin team has biobricked two non-canonical amino acid (ncAA) tRNA synthetases with their cognate tRNAs. There are now three ncAA tRNA synthetases biobricked, including one from last year's UT Austin team.

[http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Austin_Texas Group Parts - 2014]

Group Parts - 2013 (related)

These parts have been tested and characterized as part of our ncAA kit project and our Photocage project.

Related Parts and Plasmids - Not Biobricked

The 2014 UT Austin iGEM team felt quite strongly that we should make our ncAA reporter kit widely available as soon as possible. Unfortunately, this complex plasmid is not BioBrick legal, and after considerable consideration and e-mailing with Vinoo Selvarajah (iGEM HQ), we decided to include the relevant information here and be willing to share the kit plasmids (and cultures) with anyone who requests it until such time as an alternative solution is found.

Thus, until such time, we have included the information for our ncAA reporter kit here.

The two key plasmids are:

pFRYC - Reporter plasmid with linker containing tyrosine codon.

pFRY - Reporter plasmid with linker containing amber codon.

In addition, you can ask us for one or more pStG plasmids, as a control, if you like.

Additional details for using the kit and experimentals can be found on our Expanded Genetic Code Measurement Kit page.

If you have questions about this kit or would like to receive it, please contact us at utigem [at] gmail [dot) com

@@ Line 90: / Line 90: @@
 Thus, until such time, we have included the information for our [https://2014.igem.org/Team:Austin_Texas/kit#Plasmids ncAA reporter kit here].
-pFRYC - Reporter plasmid with linker containing tyrosine codon.
+The two key plasmids are:
+*pFRYC - Reporter plasmid with linker containing tyrosine codon.
-pFRY - Reporter plasmid with linker containing amber codon.
+*pFRY - Reporter plasmid with linker containing amber codon.
+In addition, you can ask us for one or more pStG plasmids, as a control, if you like.
-The two plasmids must be grown in parallel cultures.  pFRYC controls for variance in gene expression dependent on the plasmid in the specific growth conditions and the length of the fusion protein.  pFRY tests the fidelity and efficiency of the tRNA sythetase/tRNA pair.
 Additional details for [https://2014.igem.org/Team:Austin_Texas/kit#Using_The_Kit using the kit] and [https://2014.igem.org/Team:Austin_Texas/kit#Experimental_Preparation experimentals] can be found on our [https://2014.igem.org/Team:Austin_Texas/kit Expanded Genetic Code Measurement Kit page].
+If you have questions about this kit or would like to receive it, please contact us at utigem [at] gmail [dot) com