Team:Tuebingen/Notebook/Protocols/LB agar
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- | <li> Add the | + | <li> Add the reagents to 800 ml H<sub>2</sub>O</li> |
<li> Adjust pH to 7.5 with NaOH</li> | <li> Adjust pH to 7.5 with NaOH</li> | ||
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<li> Melt agar into solution in the microwave</li> | <li> Melt agar into solution in the microwave</li> | ||
- | <li> Adjust volume to | + | <li> Adjust volume to 1 L with dH<sub>2</sub>O</li> |
<li> Sterilize by autoclaving</li> | <li> Sterilize by autoclaving</li> |
Latest revision as of 19:40, 17 October 2014
Protocols
LB agar
Reagents
10 g | Bacto-tryptone |
5 g | yeast extract |
10 g | NaCl |
Procedure
- Add the reagents to 800 ml H2O
- Adjust pH to 7.5 with NaOH
- Add 15g agar
- Melt agar into solution in the microwave
- Adjust volume to 1 L with dH2O
- Sterilize by autoclaving