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Team:SYSU-China/file/Project/Notebook/Labnote/Mutation.html
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<h1>Mutation</h1> | <h1>Mutation</h1> | ||
| + | <p>May: amplify biobricks </p> | ||
| + | <p>June: clone the WT DNAQ ,recA,umuC,umuD from E.coli </p> | ||
| + | <p>July: perform the point-mutation and truncation of the four genes</p> | ||
| + | <p>August: add prefix and suffix to the genes | ||
| + | Add RBS to the genes</p> | ||
| + | <p>September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;) | ||
| + | Add promoter to construct the expression vector | ||
| + | Test the mutation rate</p> | ||
| + | <p>October: adapt the testing method and test the mutation rates | ||
| + | Prepare the biobrick for submission </p> | ||
| + | |||
<!--SYSUCHINA!--> | <!--SYSUCHINA!--> | ||
Revision as of 19:24, 17 October 2014
Mutation
May: amplify biobricks
June: clone the WT DNAQ ,recA,umuC,umuD from E.coli
July: perform the point-mutation and truncation of the four genes
August: add prefix and suffix to the genes Add RBS to the genes
September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;) Add promoter to construct the expression vector Test the mutation rate
October: adapt the testing method and test the mutation rates Prepare the biobrick for submission
