Team:Cooper Union/Notebook/Biohack May

From 2014.igem.org

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<h3>5/23/14</h3>
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   <li>Ran gel of digests from 5/22/14. Everything as it should be.</li>
   <li>Ran gel of digests from 5/22/14. Everything as it should be.</li>
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   <li>Purified phosphatase treated plasmids from 5/23/14. </li>
   <li>Purified phosphatase treated plasmids from 5/23/14. </li>

Revision as of 19:21, 17 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


5/19/14

  • Calibrated Nanodrop.
  • Nanodropped all of our samples. Very low concentration of DNA.
  • Ordered primers to PCR our g-blocks.

5/22/14

  • Struck out plates with pACYC184 with spectinomycin to grow it.
  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII/EcoRI.

5/23/14

  • Ran gel of digests from 5/22/14. Everything as it should be.
  • Purified the digested plasmids.
  • Nanodropped plasmids; results:
    Single digest I: 52.7ng/μL
    Single digest II: 64.5ng/μL
    Double digest I: 26.3ng/μL
    Double digest II: 41.9ng/μL
  • Phosphatase treated the four digested plasmids.

5/27/14

  • Purified phosphatase treated plasmids from 5/23/14.
  • Nanodropped the purified plasmids; results:
    Single digest I: 43.4ng/μL
    Single digest II: 53.6ng/μL
    Double digest I: 43.7ng/μL
    Double digest II: 16.5ng/μL

5/28/14



Projects Safety Outreach Notebook Team

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