Team:Cooper Union/Notebook/Biohack May

From 2014.igem.org

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<pre>
 
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5/19/14
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<h3>5/19/14</h3>
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Calibrated Nanodrop.
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<ul><li>Calibrated Nanodrop.</li>
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Nanodropped all of our samples. Very low concentration of DNA.
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<li>Nanodropped all of our samples. Very low concentration of DNA.</li>
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Ordered primers to PCR our g-blocks.
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<li>Ordered primers to PCR our g-blocks.</li>
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</ul>
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5/22/14:
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<h3>5/22/14</h3>
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Struck out plates with pACYC184 with spectinomycin to grow it.
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<ul>
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Digested pBR322 with HindIII.
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  <li>Struck out plates with pACYC184 with spectinomycin to grow it.</li>
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Double digested pBR322 with HindIII/EcoRI.
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  <li>Digested pBR322 with HindIII.</li>
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  <li>Double digested pBR322 with HindIII/EcoRI.</li>
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</ul>
5/23/14
5/23/14
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Ran gel of digests from 5/22/14. Everything as it should be.
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<ul>
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Purified the digested plasmids.  
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  <li>Ran gel of digests from 5/22/14. Everything as it should be.</li>
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Nanodropped plasmids; results:
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  <li>Purified the digested plasmids. </li>
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Single digest I: 52.7ng/uL
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  <li>Nanodropped plasmids; results:
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Single digest II: 64.5ng/uL
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<dl><dd>Single digest I: 52.7ng/&mu;L</dd>
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Double digest I: 26.3ng/uL
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<dd>Single digest II: 64.5ng/&mu;L</dd>
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Double digest II: 41.9ng/uL
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<dd>Double digest I: 26.3ng/&mu;L</dd>
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Phosphatase treated the four digested plasmids.  
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<dd>Double digest II: 41.9ng/&mu;L</dd>
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</dl>
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</li>
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  <li>Phosphatase treated the four digested plasmids. </li>
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</ul>
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5/27/14
5/27/14
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Purified phosphatase treated plasmids from 5/23/14.  
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<ul>
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Nanodropped the purified plasmids; results:
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  <li>Purified phosphatase treated plasmids from 5/23/14. </li>
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Single digest I: 43.4ng/uL
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  <li>Nanodropped the purified plasmids; results:
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Single digest II: 53.6ng/uL
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<dl><dd>Single digest I: 43.4ng/&mu;L</dd>
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Double digest I: 43.7ng/uL
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<dd>Single digest II: 53.6ng/&mu;L</dd>
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Double digest II: 16.5ng/uL
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<dd>Double digest I: 43.7ng/&mu;L</dd>
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<dd>Double digest II: 16.5ng/&mu;L</dd>
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</pre>  
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</dl></li>
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</ul>
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<h3>5/28/14</h3>
<h3>5/28/14</h3>
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<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="200" />
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<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
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</pre>
 

Revision as of 19:21, 17 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


5/19/14

  • Calibrated Nanodrop.
  • Nanodropped all of our samples. Very low concentration of DNA.
  • Ordered primers to PCR our g-blocks.

5/22/14

  • Struck out plates with pACYC184 with spectinomycin to grow it.
  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII/EcoRI.
5/23/14
  • Ran gel of digests from 5/22/14. Everything as it should be.
  • Purified the digested plasmids.
  • Nanodropped plasmids; results:
    Single digest I: 52.7ng/μL
    Single digest II: 64.5ng/μL
    Double digest I: 26.3ng/μL
    Double digest II: 41.9ng/μL
  • Phosphatase treated the four digested plasmids.
5/27/14
  • Purified phosphatase treated plasmids from 5/23/14.
  • Nanodropped the purified plasmids; results:
    Single digest I: 43.4ng/μL
    Single digest II: 53.6ng/μL
    Double digest I: 43.7ng/μL
    Double digest II: 16.5ng/μL

5/28/14



Projects Safety Outreach Notebook Team

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