"
Team:Pitt/Skin Probiotic/Melanin/Methods
From 2014.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
</html>{{Pitt/2014/navigationmenu}}<html> | </html>{{Pitt/2014/navigationmenu}}<html> | ||
<div class = "central_section"> | <div class = "central_section"> | ||
| - | <h2 id = "methods">Methods</h2> | + | <h2 id = "methods">Methods for MelA</h2> |
<p>Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold. </p> | <p>Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold. </p> | ||
| Line 23: | Line 23: | ||
</center> | </center> | ||
| - | |||
<h2 id = "timeline">Timeline</h2> | <h2 id = "timeline">Timeline</h2> | ||
| - | + | <center><img src = "https://static.igem.org/mediawiki/2014/c/c2/MelA_Timeline.JPG"></center> | |
| - | < | + | <br> |
| - | + | ||
| - | < | + | |
| - | + | ||
| - | + | ||
<a href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Melanin/Results"> | <a href = "https://2014.igem.org/Team:Pitt/Skin_Probiotic/Melanin/Results"> | ||
<div class = "next_page"> | <div class = "next_page"> | ||
Revision as of 18:59, 17 October 2014
Methods for MelA
Upon receiving the MelA part BBa_K193602 we transformed them into Mach1 cells, mini-prepped the plasmid and performed a diagnostic digest for the part. As can be seen in Figure # below no MelA part was observed indicating an issue with the iGEM stock of these parts. At this point we had to put this project on hold.
Figure 2. Gel of BBa_K193602 expected to contain melA with XbaI and PstI shows that the expected insert band (1896bp) is not present.
Timeline
Next Page
Previous Page
