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Team:Evry/Interlab Study/09-16-2014
From 2014.igem.org
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| - | <center>GEL | + | <center>GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.</center> |
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Revision as of 18:58, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23101:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23102:
- glycerol stock preparation from LB culture from the 14th September.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200.(18h30-1h30):
PSB1C3+E0240+J23106:
- glycerol stock preparation from LB culture from the 14th September.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200.(2h-9h):
PSB1C3+E0240+J23114:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
A pre-culture was made with one colony for each construction to make a glycerol stock.
PSB1C3+E0240+J23117 - PSB1C3+E0240+J23119:
- PCR colony of 6 colonies for each construction following protocol table 1 and 2.
GEL 2: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23117 and PSB1C3+E0240+J23119 constructions.
A pre-culture was made with one colony for each construction to make a glycerol stock.
Sep 16
