Team:Groningen/Template/MODULE/Notebook/detection/week3

From 2014.igem.org

(Difference between revisions)
(Created page with "<html> <!--Content module--> <div class="module contentmodule gridcell"> <div class="content"> <div class="wrapper"> <!-- TITLE SNIPPET START--> <div class="title"> 11 August ...")
Line 9: Line 9:
<div class="title">
<div class="title">
-
11 August - 16 August
+
August 11 - August 16
</div>
</div>
Line 18: Line 18:
<div class="text">
<div class="text">
-
K592025-B0015 was ligated to K1033225 and K1033222.
+
Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (<a href="http://parts.igem.org/Part: BBa_K1033225">BBa_K1033225</a>), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP26 (<a href="http://parts.igem.org/Part: BBa_K1033222">BBa_K1033222</a>)
 +
 
 +
 
 +
</div>
 +
<div class="hspacer">&nbsp;</div>
 +
<!-- PARAGRAPH SNIPPET END-->
 +
 
 +
<!-- PARAGRAPH SNIPPET START-->
 +
<div class="text">
 +
 
 +
Both constructs were transformed to <i>E. coli</i> DH5α
</div>
</div>
Line 27: Line 37:
<div class="text">
<div class="text">
-
Blue coloured colonies were obtained.
+
Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies
</div>
</div>
Line 36: Line 46:
<div class="text">
<div class="text">
-
The plasmids were isolated from the blue coloured transformants
+
The plasmids were isolated out of the overnight cultures
</div>
</div>
Line 45: Line 55:
<div class="text">
<div class="text">
-
The size was checked by restriction digestion
+
The size of the constructs were digested, and checked on gel
</div>
</div>

Revision as of 18:48, 17 October 2014

August 11 - August 16
 
Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (BBa_K1033225), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP26 (BBa_K1033222)
 
Both constructs were transformed to E. coli DH5α
 
Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies
 
The plasmids were isolated out of the overnight cultures
 
The size of the constructs were digested, and checked on gel
 
 
 
 

Retrieved from "http://2014.igem.org/Team:Groningen/Template/MODULE/Notebook/detection/week3"