Team:LA Biohackers/Project

Project Description

The Plan

We are using Bacillus subtilus as a backbone to incorporate the entire genome of Streptococcus thermophilus. Once incorporated, we will use the cre/lox recombination system to remove the Bacillus subtilus genome so only the Streptococcus thermophilus genome remains. This will demonstrate the use of Bacillus subtilus as a useful chassis to boot up an artificial genome.

As you may have guessed from the sly shift in tense from present to future, we did not actually succeed in transferring the entire genome of Streptococcus thermophilus into Bacillus subtilus -- yet. We have developed, and will describe, a method which we believe will enable us to do so, and the results we have obtained for the early steps in that method have been encouraging. We still believe it may be possible to accomplish our original goal, and will continue to pursue it in the weeks and months to come.

And so it begins...

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1. Overall project summary
2. Project Details
3. Materials and Methods
4. The Experiments
5. Results
6. Data analysis
7. Conclusions

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