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Team:Bordeaux/NotebookJune3
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Revision as of 18:38, 17 October 2014
2nd June
Molecular biology
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DAY TWO : Inoculate 1L SOB with 12 mL overnight culture. Grow culture at 18°C (this temperature is really important as we see a 10-fold decrease in competency when we grow them at room temperature)
- Preparation of LB medium (Agar for plates, +Ampicillin and +Chloramphenicol)
